Fig. S3
Flow tuning in vivo. (A) Experimental workflow, and a representative image of the PIV analysis in the CP. Quantification of the duration of the flow under 400 ?m/sec for each pharmacological condition in the dorsal aorta (position ISV 1, 4 and 8 from A) (n = 4 to 5 embryos per condition). (B) Experimental workflow of OT-mediated quantification of hemodynamic forces in vivo and annotated image showing trapped RBC in the DA (yellow) (see also Movie 11). Quantification and mapping of the flow force measured with OT on trapped RBCs for 3 conditions: lidocain, IBMX and control (breeding water). Values in A are mean + SD. (C and E) Image extracted from Movie 10, showing flow simulation results in the AOC model for vehicles and lidocain (C) or IBMX (E). (D and F) Velocity profiles from flow simulation at several positions for each condition (as shown in Fig.S1F). |
Reprinted from Developmental Cell, 45, Follain, G., Osmani, N., Azevedo, A.S., Allio, G., Mercier, L., Karreman, M.A., Solecki, G., Garcia Leòn, M.J., Lefebvre, O., Fekonja, N., Hille, C., Chabannes, V., Dollé, G., Metivet, T., Hovsepian, F., Prudhomme, C., Pichot, A., Paul, N., Carapito, R., Bahram, S., Ruthensteiner, B., Kemmling, A., Siemonsen, S., Schneider, T., Fiehler, J., Glatzel, M., Winkler, F., Schwab, Y., Pantel, K., Harlepp, S., Goetz, J.G., Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells, 33-52.e12, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell