Fig. 5
- ID
- ZDB-FIG-180710-37
- Publication
- Follain et al., 2018 - Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells
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Extravasation of CTCs Occurs via Flow-Dependent Endothelial Remodeling (A) Experimental workflow. (B) Extracted z projection image from 3D time-lapse analysis of the behavior of arrested TCs (red) in the CP vasculature (green) over a period of 15 hr (see also Video S13). (C) Multiple sequential images, over a period of 800 min, of the region boxed in (B) are displayed and commented (yellow arrowheads point to the location of the associated comment, left panel). (D) Multiple sequential images, over a period of 150 min, of the region boxed in (B) are displayed and commented (yellow arrowheads point to the location of the associated comment). (E) Experimental workflow and CLEM analysis of a 9-hpi vehicle-treated embryo. Extracted images of confocal analysis of extravasating TCs (red) at 9 hpi is performed. (F) Transmission electron microscopy (TEM) image of the region of interest boxed in (E), retrieved in the electron microscopy volume. A representative section is shown and color-coded for TCs (red) and vascular lumen (green) (see also Video S14). (G) 3D reconstruction of the serial section TEM performed over the entire regions of interest, depicting TCs (red) and associated ECs (green) (see also Video S14). (H) Electron tomography image extracted from 3D reconstruction over the boxed region in (F) (TC, red; ECs, green) (see also Video S14). (I) Representative images of TCs (red) and the associated vasculature (green) in 9 hpi embryos are shown. (J and K) Quantification and kinetic analysis of the mean number of events over time for vehicle- and lidocaine-treated embryos. The number of events (intravascular, pocketing, and extravascular) was quantified at 3, 9, and 24 hpi. (L) Experimental workflow and idealized representation (left). Embryos are injected with CTCs and 100-nm beads and imaged at high speed, before single-particle tracking analysis (middle). Corresponding quantification of tracks' straightness and velocity in three scenarios (open, partially closed, and closed) and in silico representation of laminar flow around arrested tumor cell (right). Values are mean ± SD. ∗p < 0.05. ns, not significant. |
Reprinted from Developmental Cell, 45, Follain, G., Osmani, N., Azevedo, A.S., Allio, G., Mercier, L., Karreman, M.A., Solecki, G., Garcia Leòn, M.J., Lefebvre, O., Fekonja, N., Hille, C., Chabannes, V., Dollé, G., Metivet, T., Hovsepian, F., Prudhomme, C., Pichot, A., Paul, N., Carapito, R., Bahram, S., Ruthensteiner, B., Kemmling, A., Siemonsen, S., Schneider, T., Fiehler, J., Glatzel, M., Winkler, F., Schwab, Y., Pantel, K., Harlepp, S., Goetz, J.G., Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells, 33-52.e12, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell