Fig. 3
- ID
- ZDB-FIG-180710-35
- Publication
- Follain et al., 2018 - Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells
- Other Figures
- (all 11)
- All Figure Page
- Back to All Figure Page
Modulating Blood Flow Forces Regulates Arrest of CTCs. (A?L) Zebrafish embryos were treated with vehicle, lidocain (A?F), or IBMX (G?L). (A and G) Experimental workflow. Heart PMA is quantified for each condition. CTL, breeding water. An additional kymograph analysis is provided. (B and H) top: images extracted from fast imaging and PIV analysis of the blood flow in the CP; region boxed in (A) and (G) (see also Video S8. Tuning the Blood Flow in the CP of 2-dpf Zebrafish Embryos, Related to Figure 3, Video S9., Video S10. Tuning and Measuring Hemodynamic Forces). Bottom: quantification of the flow profiles in the center of the DA analyzed at position ISV 8, ISV 4, and ISV1 (n = 4/5 embryos per condition). (C and I) Orthogonal flow velocity profile and quantification extracted from optical tweezing of RBCs in the DA under ISV 1 (W, wall; C, center) (see also Video S10). (D and J) Experimental workflow and representative images of the residency time of CTCs in the CP over a period of 5 min (see also Video S11). Arrowheads indicate long-lasting arrested cells (white). (E and K) Spatiotemporal quantification of the residency time of CTCs in the CP using heatmapping on a representative embryo. (F and L) Quantification of the ratio of CTCs as well as the mean arrest time over a period of 5 min for embryos treated with vehicle or the indicated drug. Norm., normalized to vehicle mean. Values are mean ± SD. ?p < 0.05, ??p < 0.01, ????p < 0.0001. |
Reprinted from Developmental Cell, 45, Follain, G., Osmani, N., Azevedo, A.S., Allio, G., Mercier, L., Karreman, M.A., Solecki, G., Garcia Leòn, M.J., Lefebvre, O., Fekonja, N., Hille, C., Chabannes, V., Dollé, G., Metivet, T., Hovsepian, F., Prudhomme, C., Pichot, A., Paul, N., Carapito, R., Bahram, S., Ruthensteiner, B., Kemmling, A., Siemonsen, S., Schneider, T., Fiehler, J., Glatzel, M., Winkler, F., Schwab, Y., Pantel, K., Harlepp, S., Goetz, J.G., Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells, 33-52.e12, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell