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Fig. S1
Validation of the efficacy of the MOs used in the study. (A-‐B) Fluorescence images showing 8-‐hpf embryos injected with the Lpar2bGFP construct (A) alone, or together with lpar2b MO (B). (C,D) Epifluorescence images of control MO-‐ or lpar2b MO2-‐injected Et(gata2:EGFP)mp189b embryos at 48 hpf. (E) The splicing-‐blocking lpar2b MO2 disrupts normal slicing of the lpar2b pre-‐mRNA as shown by RT-‐PCR. (F) Quantification of the number of NMs at 48 hpf in (C,D). *, p < 0.05 compared to control.
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Full text @ Front. Mol. Neurosci.