Fig. S3
- ID
- ZDB-FIG-180524-25
- Publication
- Sánchez-Iranzo et al., 2018 - Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration
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The transgenic tbx5a:GFP reporter line recapitulates the expression of endogenous tbx5a. a,b Sagittal section through an adult tbx5a:GFP zebrafish heart immunostained for GFP and Myosin Heavy Chain (MHC), (n=8/8). c,d mRNA in situ hybridization (ISH) with a tbx5a antisense riboprobe on the adjacent section shown in a and b. Note expression of GFP and tbx5a mRNA in the trabecular myocardium and their absence of expression in the cortical myocardium (n=8/8). b and d are zoomed views of boxed areas in a and c. e,f tbx5a ISH on a sagittal heart section of an AB wildtype adult zebrafish (n=5). g,h GFP ISH on a sagittal heart section of tbx5a:GFP zebrafish (n=4). at, atrium; ba, bulbus arteriosus; cort, cortical layer; trab, trabecular layer; wt, wildtype. Scale bars, 100 μm |