PUBLICATION

Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration

Authors
Sánchez-Iranzo, H., Galardi-Castilla, M., Minguillón, C., Sanz-Morejón, A., González-Rosa, J.M., Felker, A., Ernst, A., Guzmán-Martínez, G., Mosimann, C., Mercader, N.
ID
ZDB-PUB-180201-3
Date
2018
Source
Nature communications   9: 428 (Journal)
Registered Authors
Felker, Anastasia, Gonzalez-Rosa, Juan Manuel, Mercader Huber, Nadia, Minguillón, Carolina, Mosimann, Christian, Sánchez Iranzo, Héctor
Keywords
none
Datasets
GEO:GSE87596
MeSH Terms
  • Cell Tracking
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Embryo, Nonmammalian
  • Animals
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/metabolism
  • Cell Lineage/genetics
  • Animals, Genetically Modified
  • Organogenesis/genetics
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Regeneration/genetics*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Myocardium/cytology*
  • Myocardium/metabolism
  • Genes, Reporter
  • T-Box Domain Proteins/deficiency
  • T-Box Domain Proteins/genetics*
  • Heart Ventricles/cytology*
  • Heart Ventricles/growth & development
  • Heart Ventricles/metabolism
  • Cell Differentiation
  • Myosin Light Chains/genetics
  • Myosin Light Chains/metabolism
(all 30)
PubMed
29382818 Full text @ Nat. Commun.
Abstract
During development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a+ cells, with a small contribution from tbx5a- SHF progenitors. Notably, ablation of ventricular tbx5a+-derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells. In the adult, tbx5a expression is restricted to the trabeculae and excluded from the outer cortical layer. tbx5a-lineage tracing revealed that trabecular cardiomyocytes can switch their fate and differentiate into cortical myocardium during adult heart regeneration. We conclude that a high degree of cardiomyocyte cell fate plasticity contributes to efficient regeneration.
Genes / Markers
Figures
Figure Gallery (27 images) / 3
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cn1EtTransgenic Insertion
    cn2TgTransgenic Insertion
      cn3TgTransgenic Insertion
        cn4TgTransgenic Insertion
          cn5TgTransgenic Insertion
            cn12EtTransgenic Insertion
              cn13TgTransgenic Insertion
                cz1701TgTransgenic Insertion
                  f2TgTransgenic Insertion
                    in2TgTransgenic Insertion
                      1 - 10 of 12
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      BFPEFGBFP
                      CreEFGCre
                      DsRedEFGDsRed
                      DsRed2EFGDsRed2
                      EGFPEFGEGFP
                      LacZEFGLacZ
                      mCherryEFGmCherry
                      mRFPEFGmRFP
                      NTREFGNTR
                      TomatoEFGTomato
                      1 - 10 of 10
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                      Mapping
                      No data available
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                      Citation
                      Sánchez-Iranzo, H., Galardi-Castilla, M., Minguillón, C., Sanz-Morejón, A., González-Rosa, J.M., Felker, A., Ernst, A., Guzmán-Martínez, G., Mosimann, C., Mercader, N. (2018) Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration. Nature communications. 9:428.