PUBLICATION

Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration

Authors
Sánchez-Iranzo, H., Galardi-Castilla, M., Minguillón, C., Sanz-Morejón, A., González-Rosa, J.M., Felker, A., Ernst, A., Guzmán-Martínez, G., Mosimann, C., Mercader, N.
ID
ZDB-PUB-180201-3
Date
2018
Source
Nature communications   9: 428 (Journal)
Registered Authors
Felker, Anastasia, Gonzalez-Rosa, Juan Manuel, Mercader Huber, Nadia, Minguillón, Carolina, Mosimann, Christian, Sánchez Iranzo, Héctor
Keywords
none
Datasets
GEO:GSE87596
MeSH Terms
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Organogenesis/genetics
  • Heart Ventricles/cytology*
  • Heart Ventricles/growth & development
  • Heart Ventricles/metabolism
  • Animals
  • Regeneration/genetics*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Gene Expression Regulation, Developmental
  • Myosin Light Chains/genetics
  • Myosin Light Chains/metabolism
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/metabolism
  • Cell Tracking
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Embryo, Nonmammalian
  • Cell Differentiation
  • Genes, Reporter
  • Animals, Genetically Modified
  • Myocardium/cytology*
  • Myocardium/metabolism
  • Cell Lineage/genetics
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • T-Box Domain Proteins/deficiency
  • T-Box Domain Proteins/genetics*
PubMed
29382818 Full text @ Nat. Commun.
Abstract
During development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a+ cells, with a small contribution from tbx5a- SHF progenitors. Notably, ablation of ventricular tbx5a+-derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells. In the adult, tbx5a expression is restricted to the trabeculae and excluded from the outer cortical layer. tbx5a-lineage tracing revealed that trabecular cardiomyocytes can switch their fate and differentiate into cortical myocardium during adult heart regeneration. We conclude that a high degree of cardiomyocyte cell fate plasticity contributes to efficient regeneration.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping