FIGURE

Fig. 1S2

ID

ZDB-FIG-180222-7

Publication

Allalou et al., 2017 - Automated deep-phenotyping of the vertebrate brain

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Fig. 1S2

Registering 3D zebrafish images.

(A) Iterative shape averaging (ISA) is used to generate an average unstained reference fish (URF) for each developmental stage of interest from 5?7 wild-type embryos. Left: 2 dpf reference fish following initial alignment with a rigid transform. Right: 2 dpf reference fish after the final deformable B-spline transform. (B) Alignment of probe reference fish (PRF) to URF. Left: the URF alone (green). Center: the URF (green) with rigid alignment of PRF (magenta). Right: the URF (green) with final affine alignment of PRF (magenta). (C) Alignment of individual WISH-stained embryos to the PRF. Left: the 2 dpf PRF for tyrosine hydroxylase (th; green). Center: the PRF (green) and a second th-stained embryo (magenta) aligned using deformable registration. Areas of overlap show up as white. Right: an averaged 3D th expression pattern from 10 separate wild-type embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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