Fig. 1S1

Capillary fabrication and OPT image acquisition.

(A?C) Fabrication of a refractive index-matched tapered insertion within the glass capillary. (A) After being cleaned in Nano-Strip, the distal end of the capillary is dipped into index-matching optical adhesive and allowed to fill to approximately 5 mm by capillary action. (B) The proximal end of the capillary is attached to a miniature DC electric motor and rotated under short wavelength UV light to cure a thin outer shell of optical adhesive at the distal end. The region of the capillary where the tapered insert will be formed is protected from the UV light by an opaque shield. (C) A syringe is attached to the proximal end of the capillary and air is pumped through it at a rate of 20 mL per second. Simultaneously, long wavelength UV light is applied to completely cure the adhesive. The air flow causes a tapered surface to form in the uncured region of the optical adhesive. (D?E) Compensating for pixel-to-pixel variation in CCD sensitivity. (D) One row of pixels acquired over time prior to pixel response correction. Each row shows the sensor response over time. (E) Pixel response after correction for pixel-to-pixel variation. (F?I) Acquisition data from the OPT system showing the same WISH-stained embryo in full RGB (F) and separated into red (G), green (H), and blue (I) channels.