Fig. 5S2
3D segmentations of telencephalic expression domains. 3D segmentations of gene expression domains within and immediately adjacent to telencephalon at 2 dpf are generated in a semi-automated manner using adaptive thresholding. The gray background segmentation represents the union of telencephalic segmentations for all probes in the library and serves as an estimation for the outline for the telencephalon. Segmentations were generated from averaged 3D reconstructions. All segmentations are shown in front view (dorsal is to the top) and side view (anterior is to the left) for both wild-type and fezf2 mutant embryos. Scale bar: 100 μm. |