ZFIN ID: ZDB-FIG-171101-22
Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation. PLoS One   10:e0144982 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Long-pec

Fig. 6

FGF and Wnt/β-Catenin signalling interact to regulate osteoblast differentiation.

(A-D) Larvae were heat shocked at 48hpf, treated with SU5402 at 50hpf and fixed at 52hpf. Expression of osx is reduced after SU5402 (C) treatment and increased in hs:wnt8 larvae (B). Combined SU5402 and hs:wnt8 treatment still results in reduced expression of osx (D). (E-H) Larvae were heat shocked at 49hpf and fixed at 52hpf. Expression of osx is reduced in hs:dkk1 larvae (F) and increased in hs:fgf3 larvae (G). Expression is reduced in the combined treatment (H). (I-T) fgf3, sprouty1, sprouty4 and sef expression is inhibited by dkk1 over expression (K, N, Q, T) and activated by over expression of wnt8 (J, M, P, S). Arrowheads point to the opercle in the high magnification images to the right of each panel. Scale bars = 200μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv4 pd1Tg/+ heat shock Long-pec pharyngeal arch 3-7 ISH
fgf3 WT control Long-pec pharyngeal arch 3-7 ISH
w32Tg heat shock Long-pec pharyngeal arch 3-7 ISH
w34Tg heat shock Long-pec pharyngeal arch 3-7 ISH
il17rd WT control Long-pec pharyngeal arch 3-7 ISH
w32Tg heat shock Long-pec pharyngeal arch 3-7 ISH
w34Tg heat shock Long-pec pharyngeal arch 3-7 ISH
sp7 WT control Long-pec opercle ISH
WT chemical treatment by environment: SU5402 Long-pec opercle ISH
w32Tg heat shock Long-pec Not Detected opercle ISH
w34Tg chemical treatment by environment: SU5402 Long-pec Not Detected opercle ISH
w34Tg heat shock Long-pec opercle ISH
zf115Tg heat shock Long-pec opercle ISH
zf115Tg ; w32Tg heat shock Long-pec Not Detected opercle ISH
spry1 WT control Long-pec pharyngeal arch 3-7 ISH
w32Tg heat shock Long-pec pharyngeal arch 3-7 ISH
w34Tg heat shock Long-pec pharyngeal arch 3-7 ISH
spry4 WT control Long-pec pharyngeal arch 3-7 ISH
w32Tg heat shock Long-pec pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SU5402 Long-pec opercle sp7 expression decreased amount, abnormal
pd1Tg/+ heat shock Long-pec pharyngeal arch 3-7 etv4 expression increased amount, abnormal
w32Tg heat shock Long-pec opercle sp7 expression absent, abnormal
Long-pec pharyngeal arch 3-7 il17rd expression decreased amount, abnormal
Long-pec pharyngeal arch 3-7 spry4 expression decreased amount, abnormal
Long-pec pharyngeal arch 3-7 spry1 expression decreased amount, abnormal
Long-pec pharyngeal arch 3-7 fgf3 expression decreased amount, abnormal
w34Tg chemical treatment by environment: SU5402 Long-pec opercle sp7 expression absent, abnormal
w34Tg heat shock Long-pec opercle sp7 expression increased amount, abnormal
Long-pec pharyngeal arch 3-7 il17rd expression increased amount, abnormal
Long-pec pharyngeal arch 3-7 fgf3 expression increased amount, abnormal
Long-pec pharyngeal arch 3-7 spry1 expression increased amount, abnormal
zf115Tg heat shock Long-pec opercle sp7 expression increased amount, abnormal
zf115Tg ; w32Tg heat shock Long-pec opercle sp7 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One