FGF and Wnt/β-Catenin signalling interact to regulate osteoblast differentiation.
(A-D) Larvae were heat shocked at 48hpf, treated with SU5402 at 50hpf and fixed at 52hpf. Expression of osx is reduced after SU5402 (C) treatment and increased in hs:wnt8 larvae (B). Combined SU5402 and hs:wnt8 treatment still results in reduced expression of osx (D). (E-H) Larvae were heat shocked at 49hpf and fixed at 52hpf. Expression of osx is reduced in hs:dkk1 larvae (F) and increased in hs:fgf3 larvae (G). Expression is reduced in the combined treatment (H). (I-T) fgf3, sprouty1, sprouty4 and sef expression is inhibited by dkk1 over expression (K, N, Q, T) and activated by over expression of wnt8 (J, M, P, S). Arrowheads point to the opercle in the high magnification images to the right of each panel. Scale bars = 200μM.