ZFIN ID: ZDB-FIG-171101-23
Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation. PLoS One   10:e0144982 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Long-pec

Fig. S1

Inhibition and over activation of FGF signalling have optimal effects 3 hours after the treatment.

(A-H) Inhibition of FGF signalling, using SU5402 treatment or hs:dnfgfr1 larvae resulted in strong down regulation of pea3 and erm expression 3 hours after the treatment (B, C, F, G). Over activation of FGF signalling in hs:fgf3 larvae resulted in strong upregulation of pea3 and erm after 3 hours (D, H). (I-P) 6 hours after the treatment, inhibition of FGF signalling still resulted in slight down regulation of pea3 and erm expression (J, K, N, O) whereas expression was unchanged in hs:fgf3 larvae (L, P). Scale bar = 200μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 WT control Long-pec pharyngeal arch 3-7 ISH
WT chemical treatment by environment: SU5402 Long-pec pharyngeal arch 3-7 ISH
pd1Tg/+ heat shock Long-pec pharyngeal arch 3-7 ISH
zf115Tg heat shock Long-pec pharyngeal arch 3-7 ISH
etv5b WT control Long-pec pharyngeal arch 3-7 ISH
WT chemical treatment by environment: SU5402 Long-pec pharyngeal arch 3-7 ISH
pd1Tg/+ heat shock Long-pec pharyngeal arch 3-7 ISH
zf115Tg heat shock Long-pec pharyngeal arch 3-7 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SU5402 Long-pec pharyngeal arch 3-7 etv5b expression decreased amount, abnormal
Long-pec pharyngeal arch 3-7 etv4 expression decreased amount, abnormal
pd1Tg/+ heat shock Long-pec pharyngeal arch 3-7 etv5b expression decreased amount, abnormal
Long-pec pharyngeal arch 3-7 etv4 expression decreased amount, abnormal
zf115Tg heat shock Long-pec pharyngeal arch 3-7 etv4 expression increased amount, abnormal
Long-pec pharyngeal arch 3-7 etv5b expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One