ZFIN ID: ZDB-FIG-171101-20
Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation. PLoS One   10:e0144982 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 3

Wnt/β-Catenin signalling regulates ossification and development of mature osteoblasts.

(A-F) Continuous inhibition (hs:dkk1) or over activation (hs:wnt8) of Wnt/β-Catenin signalling from 72-120hpf. Inhibition of Wnt/β-Catenin signalling results in reduced ossification as shown by Alizarin red staining (B) whereas cartilage formation is largely unaffected (E). Overactivation of Wnt/β-Catenin signalling results in increased ossification and precocious ossification of the hyomandibula (C). (G-L) Analysis of osteoblast markers after treatment from 72-108hpf. Expression of mature osteoblast markers col1a2 and col10a1 is slightly reduced when Wnt/β-Catenin signalling is inhibited (H,K) and enhanced by increased Wnt/β-Catenin signalling (I,L). Abbreviations: hm = hyomandibula, op = opercle, te = teeth. Scale bar = 50μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col1a2 WT control Day 4 opercle ISH
w32Tg heat shock Day 4 opercle ISH
w34Tg heat shock Day 4 opercle ISH
col10a1a WT control Day 4 opercle ISH
w32Tg heat shock Day 4 opercle ISH
w34Tg heat shock Day 4 opercle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w32Tg heat shock Day 4 opercle col10a1a expression decreased amount, abnormal
Day 4 opercle col1a2 expression decreased amount, abnormal
Day 5 ceratobranchial 5 tooth ossification decreased occurrence, abnormal
Day 5 hyomandibula ossification decreased occurrence, abnormal
Day 5 opercle ossification decreased occurrence, abnormal
Day 5 ossification decreased occurrence, abnormal
w34Tg heat shock Day 4 opercle col10a1a expression increased amount, abnormal
Day 4 opercle col1a2 expression increased amount, abnormal
Day 5 ceratobranchial 5 tooth ossification increased occurrence, abnormal
Day 5 hyomandibula ossification increased occurrence, abnormal
Day 5 opercle ossification increased occurrence, abnormal
Day 5 ossification increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One