FIGURE

Fig. 3

ID

ZDB-FIG-171101-20

Publication

Felber et al., 2015 - Expression of osterix Is Regulated by FGF and Wnt/β-Catenin Signalling during Osteoblast Differentiation

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Fig. 3

Wnt/β-Catenin signalling regulates ossification and development of mature osteoblasts.

(A-F) Continuous inhibition (hs:dkk1) or over activation (hs:wnt8) of Wnt/β-Catenin signalling from 72-120hpf. Inhibition of Wnt/β-Catenin signalling results in reduced ossification as shown by Alizarin red staining (B) whereas cartilage formation is largely unaffected (E). Overactivation of Wnt/β-Catenin signalling results in increased ossification and precocious ossification of the hyomandibula (C). (G-L) Analysis of osteoblast markers after treatment from 72-108hpf. Expression of mature osteoblast markers col1a2 and col10a1 is slightly reduced when Wnt/β-Catenin signalling is inhibited (H,K) and enhanced by increased Wnt/β-Catenin signalling (I,L). Abbreviations: hm = hyomandibula, op = opercle, te = teeth. Scale bar = 50μM.

Expression Data

Genes:	col1a2 col10a1a
Fish:	WT w32Tg w34Tg
Condition:	heat shock
Anatomical Term:	opercle
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w32Tg w34Tg
Condition:	heat shock
Observed In:	ceratobranchial 5 tooth ossification hyomandibula ossification opercle opercle ossification ossification
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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