ZFIN ID: ZDB-FIG-171006-34
Du et al., 2014 - Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1. Developmental Biology   392(2):233-44 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1-4 somites to 20-25 somites

Fig. 4

Knockdown of the dvr1 gene leads to abnormal C&E movements. (A–H) Whole-mount in situ hybridization for fgf8a, dlx3b, ctsl1b, myod1 and ntla were performed in control and dvr1 moATG morphants at the indicated developmental stages. (I–P) Whole-mount in situ hybridization for ntla, dlx3b, ctsl1b, egr2b and myod1 were performed in control and dvr1 moSB morphant embryos at the indicated developmental stages. The percentage of phenotypes were as follows: (J) 17/19 (ntla), (L) 42/44 (dlx3b), 13/44 (ctsl1b), (N) 23/25 (egr2b), (P) 34/34 (myod1). Red double-headed arrows denote the width of the presumptive hindbrain in the lateral view or the width of rhombomeres 3 and 5 in the dorsal view. Green dotted lines indicate the angle between the two sides of the neural plate. Black and blue double-headed arrows show the width and length of somites in E-F and O-P, respectively. Black double-headed arrows in I and J indicate the length of the notochord. Embryos were dorsal view in left side of A-B, E-F, I-J and M-P with head to the top, animal view in C-D and K-L with head to the left, lateral view in right side of A and B with head to the top, lateral view in left side of G and H and dorsal view in right side of G and H with head to the left. (Q) The C&E defects were specifically caused by Dvr1 inhibition. Whole-mount mRNA in situ hybridization of the expression pattern of myod1 in control, dvr1 moATG-injected and dvr1 moATG plus dvr1 mRNA-injected embryos at 9-somite stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT control 1-4 somites prechordal plate ISH
WT + MO2-gdf3 standard conditions 1-4 somites prechordal plate ISH
WT + MO5-gdf3 standard conditions 1-4 somites prechordal plate ISH
dlx3b WT control 1-4 somites neural plate ISH
WT + MO2-gdf3 standard conditions 1-4 somites neural plate ISH
WT + MO5-gdf3 standard conditions 1-4 somites neural plate ISH
egr2b WT control 1-4 somites hindbrain neural keel ISH
WT + MO5-gdf3 standard conditions 1-4 somites hindbrain neural keel ISH
fgf8a WT control Bud hindbrain neural keel ISH
WT + MO2-gdf3 standard conditions Bud hindbrain neural keel ISH
myod1 WT control 5-9 somites somite ISH
WT + MO2-gdf3 standard conditions 5-9 somites somite ISH
WT + MO5-gdf3 standard conditions 5-9 somites somite ISH
tbxta WT control Bud notochord ISH
20-25 somites notochord ISH
WT + MO2-gdf3 standard conditions 20-25 somites notochord ISH
WT + MO5-gdf3 standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-gdf3 standard conditions 1-4 somites convergent extension involved in gastrulation decreased occurrence, abnormal
Bud convergent extension involved in gastrulation decreased occurrence, abnormal
Bud hindbrain neural keel increased width, abnormal
1-4 somites neural plate increased width, abnormal
1-4 somites prechordal plate mislocalised posteriorly, abnormal
5-9 somites convergent extension involved in gastrulation decreased occurrence, abnormal
5-9 somites somite antero-posteriorly flattened, abnormal
5-9 somites somite distended, abnormal
20-25 somites convergent extension involved in gastrulation decreased occurrence, abnormal
20-25 somites notochord curved, abnormal
WT + MO5-gdf3 standard conditions 1-4 somites convergent extension involved in gastrulation decreased occurrence, abnormal
Bud convergent extension involved in gastrulation decreased occurrence, abnormal
1-4 somites hindbrain neural keel increased width, abnormal
Bud notochord decreased length, abnormal
1-4 somites prechordal plate mislocalised posteriorly, abnormal
1-4 somites rhombomere increased width, abnormal
5-9 somites convergent extension involved in gastrulation decreased occurrence, abnormal
5-9 somites somite antero-posteriorly flattened, abnormal
5-9 somites somite distended, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 392(2), Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H., Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1, 233-44, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.