Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1
- Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H.
- Developmental Biology 392(2): 233-44 (Journal)
- Registered Authors
- Chen, Yi, Deng, Min, Dong, Mei, Jin, Yi, Liu, Ting Xi, Pan, Weijun, Xu, Peng-Fei
- TGF-β superfamily, Zebrafish, convergence and extension, dvr1, histone methylation, setdb2
- MeSH Terms
- Blotting, Western
- Cell Movement/physiology*
- Fluorescent Antibody Technique
- Gene Expression Regulation, Developmental/physiology*
- Gene Knockdown Techniques
- Histone-Lysine N-Methyltransferase/metabolism
- Histone-Lysine N-Methyltransferase/physiology*
- In Situ Hybridization
- Microarray Analysis
- Real-Time Polymerase Chain Reaction
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology*
- 24892953 Full text @ Dev. Biol.
Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H. (2014) Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1. Developmental Biology. 392(2):233-44.
As the primary driving forces of gastrulation, convergence and extension (C&E) movements lead to a medio-lateral narrowing and an anterior-posterior elongation of the embryonic body axis. Histone methylation as a post-translational modification plays a critical role in early embryonic development, but its functions in C&E movements remain largely unknown. Here, we show that the setdb2-dvr1 transcriptional cascade plays a critical role in C&E movements during zebrafish gastrulation. Knockdown of Setdb2, a SET domain-containing protein possessing a potential histone H3K9 methyltransferase activity, induced abnormal C&E movements, resulting in anterior-posterior shortening and medio-lateral expansion of the embryonic axis, as well as abnormal notochord cell polarity. Furthermore, we found that Setdb2 functions through fine-tuning the expression of dvr1, a ligand of the TGF-β superfamily, to an appropriate level to ensure proper C&E movements in a non-cell-autonomous manner. In addition, both overexpression and knockdown of Dvr1 at the one-cell stage resulted in defects at epiboly and C&E. These data demonstrate that Setdb2 is a novel regulator for C&E movements and acts by modulating the expression level of dvr1, suggesting that Dvr1 acts as a direct and essential mediator for C&E cell movements.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes