Fig. 6

The setdb2-dvr1 transcriptional cascade regulates C&E cell movements in a non-cell-autonomous manner. (A-B) Representative images of transplantation for analysis of C&E cell movements at the shield stage. Red and green cells are those labeled with Rhodamine-dextran or fluorescein-dextran in donor embryos, respectively. (C-D) Dorsal or lateral mesendodermal cells derived from wild-type and setdb2-mo donor embryos, respectively, were transplanted into the corresponding regions of wild-type host embryos at the shield stage and imaged at 11 hpf. (E-F) Dorsal or lateral mesendodermal cells derived from wild-type and setdb2-mo donor embryos, respectively, were transplanted into the corresponding regions of setdb2 mo-injected host embryos at the shield stage and imaged at 11 hpf. (G-H) Dorsal or lateral mesendodermal cells derived from wild-type and dvr1 mo^SB donor embryos, respectively, were transplanted into the corresponding regions of wild-type host embryos at the shield stage and imaged at 11 hpf. (I-J) Dorsal or lateral mesendodermal cells derived from wild-type and dvr1 mo^SB donor embryos, respectively, were transplanted into the corresponding regions of dvr1 mo^SB-injected host embryos at the shield stage and imaged at 11 hpf. The percentages of phenotypes were as follows: (C) 7/9, (D) 6/6, (E) 7/8, (F) 9/9, (G) 8/10, (H) 6/6, (I) 8/8, (J) 9/10. The angles in C, E, G and I indicate the anterior migration of dorsal mesendodermal cells after transplantation. The white arrows in D, F, H and J indicate the dorsal migration of lateral mesendodermal cells after transplantation. All embryos were lateral views with head to the top.