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ZIRC
ZFIN ID: ZDB-FIG-171004-9
Demy et al., 2017 - Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues. Journal of Cell Science   130(17):2797-2807 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

Macrophages are produced normally but do not colonize the CNS in moonshine mutants. (A–C) In vivo images of whole monNQ039Tg(mpeg1:mCherryF) siblings (Sib) (A, upper) and mutants (Mut) (A, lower) at 48 hpf, and dorsal view (rostral up) of the midbrain of siblings (B) and mutants (C) at 72 hpf. (D) Counts (Nb, number) at 48 hpf show that monNQ039Tg(mpeg1:mCherryF) mutant embryos have significantly less mCherry-positive macrophages in the head despite having a similar total number of macrophages to that in the siblings. ISH, whole-mount in situ hybridization. (E–I) Whole mount in situ hybridization for Csfr1a mRNA reveals numerous macrophages in the retina at 48 hpf (E) and midbrain at 72 hpf (G) in the siblings, whereas very few such cells can be detected in these organs in the mutants (F,H). (I) Counting of these cells over time reveals a lower number in the mutant CNS at all stages. Error bars show mean±s.e.m. **P<0.01; ***P<0.001; ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra TU control Long-pec retina macrophage ISH
Protruding-mouth midbrain macrophage ISH
trim33t30813/t30813(TU) control Long-pec retina macrophage ISH
Protruding-mouth midbrain macrophage ISH
mCherry ump2Tg control Long-pec head macrophage IFL
Long-pec whole organism macrophage IFL
Protruding-mouth head macrophage IFL
ump2Tg ; trim33t30813/t30813 control Long-pec head macrophage IFL
Long-pec whole organism macrophage IFL
Protruding-mouth head macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
trim33t30813/t30813(TU) control Long-pec central nervous system macrophage decreased amount, abnormal
Long-pec head macrophage decreased amount, abnormal
Long-pec macrophage migration decreased process quality, abnormal
Long-pec retina macrophage decreased amount, abnormal
Long-pec retina macrophage csf1ra expression decreased amount, abnormal
Protruding-mouth central nervous system macrophage decreased amount, abnormal
Protruding-mouth head macrophage decreased amount, abnormal
Protruding-mouth macrophage migration decreased process quality, abnormal
Protruding-mouth midbrain macrophage decreased amount, abnormal
Protruding-mouth midbrain macrophage csf1ra expression decreased amount, abnormal
trim33t30813/t30813(TU) standard conditions Pec-fin central nervous system macrophage decreased amount, abnormal
Pec-fin head macrophage decreased amount, abnormal
Pec-fin macrophage migration decreased process quality, abnormal
ump2Tg ; trim33t30813/t30813 control Long-pec head macrophage decreased amount, abnormal
Long-pec head macrophage mCherry expression decreased amount, abnormal
Long-pec macrophage migration decreased process quality, abnormal
Long-pec whole organism macrophage mCherry expression amount, ameliorated
Long-pec whole organism macrophage amount, normal
Protruding-mouth head macrophage mCherry expression decreased amount, abnormal
Protruding-mouth macrophage migration decreased process quality, abnormal
Protruding-mouth midbrain macrophage decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.