ZFIN ID: ZDB-FIG-171004-16
Demy et al., 2017 - Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues. Journal of Cell Science   130(17):2797-2807 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Protruding-mouth

Fig. S1

MonNQ039 mutants and Trim33 morphants share phenotypic traits with monTB222 mutants. (A-B') In vivo images of DsRed+ erythroid cells in monNQ039Tg(gata1:DsRed) embryos at 48 hpf in the yolk sac (A,B) or tail (A',B') of sibling (A, A') and mutant (B,B') embryos. Circulating erythrocytes can be seen in the siblings heart (a, purple arrowhead) and duct of Cuvier (A, blue arrowhead), as well as in their caudal artery (A', purple arrowhead) and caudal vein (A', blue arrowhead), but not in the moonshine mutant (B-B'). (C,D) Whole mount O-dianisidine staining of hemoglobin-loaded erythrocytes at 72 hpf in the anterior region of sibling (C) and mutant (D) monNQ039/Tü larvae. Erythrocytes can be seen in the duct of Cuvier (blue arrowhead) and heart (purple arrowhead) of sibling but not mutant larvae. (E-F) In vivo video-enhanced Nomarski microscopy at 18 hpf reveals that the Posterior Blood Island of a monNQ039 mutant (F) is full of apoptotic bodies instead of erythroid progenitors as seen in the sibling (E). (G-H') In vivo images of whole (G,H) or PBI (G',H') of Tg(TBP:Gal4;UAS:AnV-YFP) (G,H) or Tg(TBP:Gal4/UAS:AnV-YFP; gata1a:DsRed) (G',H') embryos at 26 hpf. Embryos injected with the Trim33 morpholino (H,H’) have a lot of apoptotic cells (green channel) corresponding to erythroid progenitors (red channel), unlike in the control embryos (G,G'). (I-J') MonNQ039 mutants (J-J') display a smaller and more irregularly shaped caudal fin than their siblings (I), as shown by the dotted line. Some mutants also have more iridophores (J', blue arrowhead). Ctrl, control; Mo, morphant; Mut, mutant; Sib, sibling.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f12Tg; f13Tg + MO4-trim33 control Prim-5 whole organism apoptotic process increased occurrence, abnormal
f12Tg; f13Tg; sd2Tg + MO4-trim33 control Prim-5 erythroid progenitor cell apoptotic, abnormal
trim33t30813/t30813 control 14-19 somites blood island lacks all parts of type erythroid progenitor cell, abnormal
14-19 somites blood island apoptotic process increased occurrence, abnormal
Protruding-mouth common cardinal vein lacks all parts of type nucleate erythrocyte, abnormal
Protruding-mouth heart lacks all parts of type nucleate erythrocyte, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.