ZFIN ID: ZDB-FIG-171004-17
Demy et al., 2017 - Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues. Journal of Cell Science   130(17):2797-2807 Full text @ J. Cell Sci.
Anatomical Term:
Stage: Prim-25

Fig. S2

Macrophages do not colonize the CNS in monTB222 mutants and Trim33 morphants. (A-B) Csfr1a ISH at 36 hpf shows that the initial primitive macrophage production and spreading in the monTB22 mutant (B) are similar to the sibling (A). (C-D') In vivo images of retinas (C,D) or midbrain (C'-D') of 3 days old Tg(HuC:GFP; mpeg1:Gal4; UAS:NfsB-mCherry) embryos injected (D-D') or not (C,C') with the Trim33 morpholino. Trim33 KO (D,D') causes macrophage depletion in both CNS compartments. (E-G) Lplastin whole-mount immunodetection (combined with DAPI detection of cell nuclei) in monTB222/Tü siblings (E,E') and mutants (F,F') reveals numerous leukocytes (macrophages) in the retinas (E) and midbrain (E') of 3 days old siblings, whereas there are only a few in the mutants. Counting of these cells every 6 hrs from 30 to 66 hpf reveals that monTB222 mutants have less leukocytes in the CNS at all stages. Ctrl, control; Mo, morphant; Mut, mutant; nb, number; Sib, sibling. **, P value <0.01; ***, P value < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra TU control Prim-25 macrophage ISH
trim33tb222b/tb222b control Prim-25 macrophage ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.