ZFIN ID: ZDB-FIG-170606-34
Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish. Developmental Biology   425(2):130-141 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 8

wnt8a knockdown reduces the number of gata1+ and spi1+ blood progenitors. Top panels of each treatment show flat mounted dorsal views of the trunks of 16 hpf embryos stained for gata1, spi1 and kdrl after the indicated treatments. Bottom panels are higher magnification views of the corresponding panel above.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gata1a TU control 14-19 somites erythroid lineage cell ISH
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites erythroid lineage cell ISH
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites erythroid lineage cell ISH
kdrl TU control 14-19 somites endothelial cell ISH
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites endothelial cell ISH
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites endothelial cell ISH
spi1b TU control 14-19 somites common myeloid progenitor ISH
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites common myeloid progenitor ISH
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites common myeloid progenitor ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites common myeloid progenitor spi1b expression increased amount, abnormal
14-19 somites endothelial cell migration process quality, abnormal
14-19 somites erythroid lineage cell gata1a expression increased amount, abnormal
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites common myeloid progenitor spi1b expression decreased amount, abnormal
14-19 somites erythroid lineage cell gata1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.