ZFIN ID: ZDB-FIG-170606-33
Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish. Developmental Biology   425(2):130-141 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7

Effect of wnt8a knockdown on inter-nuclear distance. A) Lateral views of 24 hpf embryos stained for slc4a4 (red) and slc12a3 (purple). Black arrows identify the anterior end of the slc12a3+ DL segment. B) Panels show lateral views of the pronephric tubule (proximal left, distal right) stained for Hnf1b to identify the nucleus of renal epithelial cells in response to wnt8a knockdown and/or HUA treatment. Histogram shows the average inter-nuclear distances of cells in the proximal region of the pronephros for each treatment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc4a4a TU control Prim-5 pronephric tubule proximal region ISH
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule proximal region ISH
TU + MO1-wnt8a + MO2-wnt8a control Prim-5 pronephric tubule proximal region ISH
slc12a3 TU control Prim-5 pronephric tubule distal region ISH
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule distal region ISH
TU + MO1-wnt8a + MO2-wnt8a control Prim-5 pronephric tubule distal region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-hnf1b IHC TU control Prim-5 pronephric tubule distal region
IHC Prim-5 pronephric tubule proximal region
IHC TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule distal region
IHC Prim-5 pronephric tubule proximal region
IHC TU + MO1-wnt8a + MO2-wnt8a control Prim-5 pronephric tubule distal region
IHC Prim-5 pronephric tubule proximal region
IHC TU + MO1-wnt8a + MO2-wnt8a chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule distal region
IHC Prim-5 pronephric tubule proximal region
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule cell increased distance pronephric tubule cell, abnormal
Prim-5 pronephric tubule distal region slc12a3 expression decreased amount, abnormal
Prim-5 pronephric tubule distal region slc12a3 expression decreased distribution, abnormal
TU + MO1-wnt8a + MO2-wnt8a control Prim-5 pronephric tubule cell increased distance pronephric tubule cell, abnormal
Prim-5 pronephric tubule distal region slc12a3 expression decreased amount, abnormal
Prim-5 pronephric tubule distal region slc12a3 expression decreased distribution, abnormal
TU + MO1-wnt8a + MO2-wnt8a chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea Prim-5 pronephric tubule cell increased distance pronephric tubule cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.