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ZFIN ID: ZDB-FIG-170606-29
Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish. Developmental Biology   425(2):130-141 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 3

Effects of wnt8a knockdown and BIO on renal progenitor cell number. A-G) Left panels show flat mounted dorsal views of the trunks of 16 hpf embryos stained for pax2a transcripts and treated as indicated. Right panels show close-up views Hnf1b+ nuclei in the proximal pronephros (location indicated by the red dotted box in the embryo in A). Scale bar represents 50 ┬Ám. H) Scatter-graph showing empirical counts of Hnf1b+ nuclei after the various treatments shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a TU control 14-19 somites pronephros ISH
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephros ISH
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: 6-bromoindirubin-3'-oxime, chemical treatment by environment: hydroxyurea 14-19 somites pronephros ISH
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea 14-19 somites pronephros ISH
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites pronephros ISH
TU + MO1-wnt8a + MO2-wnt8a chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephros ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-hnf1b IHC TU control 14-19 somites pronephric tubule
IHC TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephric tubule
IHC TU chemical treatment by environment: aphidicolin, chemical treatment by environment: 6-bromoindirubin-3'-oxime, chemical treatment by environment: hydroxyurea 14-19 somites pronephric tubule
IHC TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea 14-19 somites pronephric tubule
IHC TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites pronephric tubule
IHC TU + MO1-wnt8a + MO2-wnt8a chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephric tubule
Phenotype Details
Fish Conditions Stage Phenotype
TU chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephric tubule Ab1-hnf1b labeling increased amount, abnormal
14-19 somites pronephric tubule proximal region has extra parts of type cell, abnormal
14-19 somites pronephros pax2a expression increased amount, abnormal
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: 6-bromoindirubin-3'-oxime, chemical treatment by environment: hydroxyurea 14-19 somites pronephric tubule Ab1-hnf1b labeling decreased amount, abnormal
14-19 somites pronephric tubule proximal region has fewer parts of type cell, abnormal
TU chemical treatment by environment: aphidicolin, chemical treatment by environment: hydroxyurea 14-19 somites pronephric tubule Ab1-hnf1b labeling decreased amount, abnormal
14-19 somites pronephric tubule proximal region has fewer parts of type cell, abnormal
TU + MO1-wnt8a + MO2-wnt8a control 14-19 somites pronephric tubule Ab1-hnf1b labeling decreased amount, abnormal
14-19 somites pronephric tubule proximal region has fewer parts of type cell, abnormal
14-19 somites pronephros pax2a expression decreased amount, abnormal
TU + MO1-wnt8a + MO2-wnt8a chemical treatment by environment: 6-bromoindirubin-3'-oxime 14-19 somites pronephric tubule Ab1-hnf1b labeling amount, ameliorated
14-19 somites pronephric tubule proximal region has number of cell, ameliorated
14-19 somites pronephros pax2a expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.