ZFIN ID: ZDB-FIG-170606-30
Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish. Developmental Biology   425(2):130-141 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 4

Effect of wnt8a knockdown on apoptosis. A) Left panels show dorsal views of the posterior region of 16 hpf embryos stained for activated Caspase3 and treated as indicated. Dashed line box indicates the region used to count the number of Caspase3+ cells. Scatter-graph on the right illustrates the counts for Caspase3+ cells. B) Panels show dorsal views of the posterior region of flat mounted embryos stained for slc12a3 transcripts at 16 hpf and treated as indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
slc12a3 TU standard conditions 14-19 somites pronephric tubule ISH
TU + MO1-wnt8a + MO2-wnt8a standard conditions 14-19 somites pronephric tubule ISH
TU + MO1-wnt8a + MO2-wnt8a + MO4-tp53 standard conditions 14-19 somites pronephric tubule ISH
TU + MO4-tp53 standard conditions 14-19 somites pronephric tubule ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-casp3 IHC TU standard conditions 14-19 somites whole organism posterior region
IHC TU + MO1-wnt8a + MO2-wnt8a standard conditions 14-19 somites whole organism posterior region
IHC TU + MO1-wnt8a + MO2-wnt8a + MO4-tp53 standard conditions 14-19 somites whole organism posterior region
IHC TU + MO4-tp53 standard conditions 14-19 somites whole organism posterior region
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-wnt8a + MO2-wnt8a standard conditions 14-19 somites pronephric tubule slc12a3 expression decreased amount, abnormal
TU + MO1-wnt8a + MO2-wnt8a + MO4-tp53 standard conditions 14-19 somites apoptotic process decreased occurrence, abnormal
14-19 somites pronephric tubule slc12a3 expression decreased amount, abnormal
14-19 somites whole organism posterior region ab1-casp3 labeling decreased amount, abnormal
TU + MO4-tp53 standard conditions 14-19 somites apoptotic process decreased occurrence, abnormal
14-19 somites whole organism posterior region ab1-casp3 labeling decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.