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ZFIN ID: ZDB-FIG-170602-11
Rong et al., 2017 - Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos. Development (Cambridge, England)   144(9):1687-1697 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Dome to 90%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Dome to 90%-epiboly

Fig. 2

Maternal loss of gpx4b promotes dorsal organizer development and depletion of gpx4b increases Wnt/β-catenin activity. (A) Expression of dorsal organizer markers chd and gsc mRNA in each indicated group of embryos at 4.3 hpf, as assessed by whole-mount in situ hybridization. Upper panels are animal pole views with dorsal towards the right. Arrows indicate the edges of the chd and gsc mRNA expression domains. Lower panels are dorsal views with animal pole upwards. The frequency of embryos with the indicated patterns is shown in the bottom right corner of each group. (B) Quantification of the arc of marker expression shown in A. (C) The expression levels of boz, chd and sqt mRNA in each indicated group of embryos at 4.3 hpf, as analyzed by qRT-PCR. (D) Expression of direct zygotic Wnt markers cdx4, sp5l and tbx6 in MZallele1 mutants and wild-type embryos at 80% or 90% epiboly stage (9 hpf). Asterisks indicate the edges of the indicated mRNA expression domains. Lateral views with dorsal towards the right and animal pole upwards. (E) The mRNA expression levels of indicated zygotic Wnt direct target genes in wild-type and MZallele1 mutant embryos at 8 hpf analyzed by qRT-PCR. (F) MZallele1 mutants show increased endogenous Wnt signaling. Values are means±s.e.m. (n=3). *P<0.05; **P<0.01; ***P<0.001. Unpaired t-test, two-tailed. Scale bars: 200 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 gpx4bouc1/ouc1 standard conditions 75%-epiboly whole organism RTPCR
ccnd1 gpx4bouc1/ouc1 standard conditions 75%-epiboly whole organism RTPCR
cdx4 TU standard conditions 75%-epiboly anatomical structure ISH
gpx4bouc1/ouc1 standard conditions 75%-epiboly anatomical structure ISH
75%-epiboly whole organism RTPCR
chrd TU standard conditions Dome whole organism dorsal region ISH
gpx4bouc1/ouc1 standard conditions Dome whole organism RTPCR
Dome whole organism dorsal region ISH
gpx4bouc1 standard conditions Dome whole organism RTPCR
Dome whole organism dorsal region ISH
dharma gpx4bouc1/ouc1 standard conditions Dome whole organism RTPCR
gpx4bouc1 standard conditions Dome whole organism RTPCR
gsc TU standard conditions Dome whole organism dorsal region ISH
gpx4bouc1/ouc1 standard conditions Dome whole organism dorsal region ISH
gpx4bouc1 standard conditions Dome whole organism dorsal region ISH
ndr1 gpx4bouc1/ouc1 standard conditions Dome whole organism RTPCR
gpx4bouc1 standard conditions Dome whole organism RTPCR
sp5l TU standard conditions 75%-epiboly anatomical structure ISH
gpx4bouc1/ouc1 standard conditions 75%-epiboly anatomical structure ISH
75%-epiboly whole organism RTPCR
tbx6 TU standard conditions 90%-epiboly anatomical structure ISH
gpx4bouc1/ouc1 standard conditions 90%-epiboly anatomical structure ISH
vent gpx4bouc1/ouc1 standard conditions 75%-epiboly whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gpx4bouc1/ouc1 standard conditions Dome whole organism chrd expression increased amount, abnormal
Dome whole organism ndr1 expression increased amount, abnormal
Dome whole organism dharma expression increased amount, abnormal
Dome whole organism dorsal region gsc expression increased distribution, abnormal
Dome whole organism dorsal region chrd expression increased distribution, abnormal
75%-epiboly anatomical structure cdx4 expression increased amount, abnormal
75%-epiboly anatomical structure sp5l expression increased amount, abnormal
75%-epiboly whole organism cdx4 expression increased amount, abnormal
75%-epiboly whole organism ccnd1 expression increased amount, abnormal
75%-epiboly whole organism axin2 expression increased amount, abnormal
75%-epiboly whole organism vent expression increased amount, abnormal
75%-epiboly whole organism sp5l expression increased amount, abnormal
75%-epiboly Wnt signaling pathway increased occurrence, abnormal
90%-epiboly anatomical structure tbx6 expression increased amount, abnormal
gpx4bouc1 standard conditions Dome whole organism ndr1 expression increased amount, abnormal
Dome whole organism dharma expression increased amount, abnormal
Dome whole organism chrd expression increased amount, abnormal
Dome whole organism dorsal region chrd expression increased distribution, abnormal
Dome whole organism dorsal region gsc expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development