ZFIN ID: ZDB-FIG-170602-17
Rong et al., 2017 - Glutathione peroxidase 4 inhibits Wnt/β-catenin signaling and regulates dorsal organizer formation in zebrafish embryos. Development (Cambridge, England)   144(9):1687-1697 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 1-cell to Day 4

Fig. S1

Amino acid sequence alignment of GPX4/Gpx4 and spatiotemporal expression pattern of zebrafish gpx4b. (A) Amino acid sequence alignment of human, mouse, and zebrafish GPX4/Gpx4. Identical amino acids are indicated in black and similar amino acids are indicated in grey. The Sec residue (U) is indicated with an asterisk and highlighted in red. The accession numbers are as follows: human GPX4 NP_002076.2, mouse GPX4 NP_032188.3, zebrafish Gpx4b NP_001025241.2, zebrafish Gpx4a NP_001007283.2. (B) RT-PCR analysis of zebrafish gpx4b mRNA at the indicated embryonic stages. Numbers indicate different developmental stages as hours post fertilization (hpf). β-actin was used as internal control. +, positive control; -, negative control. (C) Whole-mount in situ hybridization analysis of zebrafish gpx4b mRNA at the indicated stages. Panels are dorsal, top, or lateral views with animal pole up or anterior to the left. Scale bars = 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gpx4b TU standard conditions 1-cell whole organism ISH
1-cell whole organism RTPCR
4-cell whole organism ISH
64-cell whole organism RTPCR
64-cell whole organism ISH
Sphere whole organism RTPCR
Shield whole organism RTPCR
Shield whole organism ISH
5-9 somites whole organism ISH
5-9 somites whole organism RTPCR
14-19 somites whole organism ISH
Prim-5 whole organism RTPCR
Prim-5 whole organism ISH
Long-pec whole organism RTPCR
Long-pec whole organism ISH
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development