ZFIN ID: ZDB-FIG-170531-8
Miyake et al., 2017 - Brorin is required for neurogenesis, gliogenesis, and commissural axon guidance in the zebrafish forebrain. PLoS One   12:e0176036 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 7

Specification of GABAergic interneurons and oligodendrocytes, and astroglial development in brorin morphants.

The expression of ascl1a (A, B), gad1 (C, D), ngn1 (E, F), plp (G, H), and glula (I, J) in wild-type embryos (A, C, E, G, I) and brorin morphants (B, D, F, H, J) is displayed at the indicated stages. di, diencephalon; pa, pallial telencephalon; poc, postoptic commissure; sp, subpallial telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ascl1a WT standard conditions Prim-5 diencephalon GABAergic neuron ISH
Prim-5 ventral telencephalon GABAergic neuron ISH
WT + MO1-vwc2 standard conditions Prim-5 diencephalon GABAergic neuron ISH
Prim-5 ventral telencephalon GABAergic neuron ISH
gad1b WT standard conditions Prim-5 nucleus of the tract of the postoptic commissure GABAergic neuron ISH
Prim-5 ventral telencephalon GABAergic neuron ISH
WT + MO1-vwc2 standard conditions Prim-5 nucleus of the tract of the postoptic commissure GABAergic neuron ISH
Prim-5 ventral telencephalon GABAergic neuron ISH
glula WT standard conditions Protruding-mouth brain astrocyte ISH
WT + MO1-vwc2 standard conditions Protruding-mouth brain astrocyte ISH
neurog1 WT standard conditions Prim-5 diencephalon ventral region ISH
Prim-5 dorsal telencephalon ISH
WT + MO1-vwc2 standard conditions Prim-5 diencephalon ventral region ISH
Prim-5 dorsal telencephalon ISH
plp1a WT standard conditions Day 4 brain oligodendrocyte ISH
WT + MO1-vwc2 standard conditions Day 4 brain oligodendrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-vwc2 standard conditions Prim-5 diencephalon GABAergic neuron ascl1a expression decreased amount, abnormal
Prim-5 diencephalon neuron differentiation decreased process quality, abnormal
Prim-5 diencephalon ventral region neurog1 expression decreased amount, abnormal
Prim-5 forebrain GABAergic neuron differentiation decreased process quality, abnormal
Prim-5 nucleus of the tract of the postoptic commissure GABAergic neuron gad1b expression decreased amount, abnormal
Prim-5 ventral telencephalon GABAergic neuron gad1b expression decreased amount, abnormal
Prim-5 ventral telencephalon GABAergic neuron ascl1a expression decreased amount, abnormal
Prim-5 ventral telencephalon neuron differentiation decreased process quality, abnormal
Protruding-mouth brain astrocyte glula expression increased amount, abnormal
Protruding-mouth brain astrocyte development increased occurrence, abnormal
Day 4 brain oligodendrocyte plp1a expression decreased amount, abnormal
Day 4 brain oligodendrocyte development decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One