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ZFIN ID: ZDB-FIG-170517-36
Anelli et al., 2017 - Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 45-89
PHENOTYPE:
Fish:
Observed In:
Stage: Days 45-89

Fig. 3 S1

Gene expression changes induced by BRAFV600E in larval thyrocytes.

(A) qPCR analysis of select up-regulated genes; data are represented as fold change of tg-BRAFV600E-TOM compared to tg-TOM and are normalized using gapdh as a control. (B) GSEA hallmark gene sets significantly enriched in tg-BRAFV600E-TOM (top 10 signatures, FDR < 25%, nominal p-value<5%). (C) GSEA enrichment plot of ERK signature using RNAseq data generated from sorted zebrafish thyrocytes as described. (D) Heat-map of ERK signature genes (n = 37) differentially expressed in tg-BRAFV600E-TOM sorted thyrocytes compared to tg-TOM. (E) Validation of differential expression of select ERK signature genes using qPCR, data are normalized using gapdh as a control. (F) E-cadherin immunostaining in tg-TOM and tg-BRAFV600E-TOM larvae at 5dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 wcm101Tg control Days 45-89 thyroid follicle RTPCR
egr1 wcm101Tg control Days 45-89 thyroid follicle RTPCR
fosab wcm101Tg control Days 45-89 thyroid follicle RTPCR
her6 wcm101Tg control Days 45-89 thyroid follicle RTPCR
spred2a wcm101Tg control Days 45-89 thyroid follicle RTPCR
twist3 wcm101Tg control Days 45-89 thyroid follicle RTPCR
wnt11 wcm101Tg control Days 45-89 thyroid follicle RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wcm101Tg control Days 45-89 thyroid follicle twist3 expression increased amount, abnormal
Days 45-89 thyroid follicle fosab expression increased amount, abnormal
Days 45-89 thyroid follicle spred2a expression increased amount, abnormal
Days 45-89 thyroid follicle axin2 expression increased amount, abnormal
Days 45-89 thyroid follicle her6 expression increased amount, abnormal
Days 45-89 thyroid follicle egr1 expression increased amount, abnormal
Days 45-89 thyroid follicle wnt11 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife