FIGURE

Fig. 3 S1

ID

ZDB-FIG-170517-36

Publication

Anelli et al., 2017 - Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3 S1

Gene expression changes induced by BRAF^V600E in larval thyrocytes.

(A) qPCR analysis of select up-regulated genes; data are represented as fold change of tg-BRAF^V600E-TOM compared to tg-TOM and are normalized using gapdh as a control. (B) GSEA hallmark gene sets significantly enriched in tg-BRAF^V600E-TOM (top 10 signatures, FDR < 25%, nominal p-value<5%). (C) GSEA enrichment plot of ERK signature using RNAseq data generated from sorted zebrafish thyrocytes as described. (D) Heat-map of ERK signature genes (n = 37) differentially expressed in tg-BRAF^V600E-TOM sorted thyrocytes compared to tg-TOM. (E) Validation of differential expression of select ERK signature genes using qPCR, data are normalized using gapdh as a control. (F) E-cadherin immunostaining in tg-TOM and tg-BRAF^V600E-TOM larvae at 5dpf.

Expression Data

Genes:	axin2 egr1 fosab her6 spred2a twist3 wnt11
Fish:	wcm101Tg
Anatomical Term:	thyroid follicle
Stage:	Days 45-89

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wcm101Tg
Observed In:	thyroid follicle
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife