FIGURE

Fig. 1

ID
ZDB-FIG-170517-32
Publication
Anelli et al., 2017 - Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression
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Fig. 1

BRAFV600E expression in thyrocytes disrupts follicle structure.

(A) Schematic diagram of the constructs used to create transgenic lines, containing a specific zebrafish thyroglobulin promoter (zf tg prom) driving expression of either human BRAFV600E or TdTomato fluorophore. (B?C) Brightfield micrographs overlayed on to an epifluorescent micrograph of a live 5 dpf tg-TOM and tg-BRAFV600E-TOM larvae. (D?E) Representative confocal images of 5 dpf fixed tg-TOM and tg-BRAFV600E-TOM larvae immunostained with anti-T4 antibody; white arrows indicate T4-positive follicles. (F) Quantification of the total follicle number in tg-TOM and tg-BRAFV600E-TOM larvae (n = 20). (G) Measurement of follicle diameter in tg-TOM and tg-BRAFV600E-TOM (n = 45 and n = 30, respectively). ***p?0.001, Student's two-tailed t-test.
Expression Data
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Day 5

Phenotype Detail
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