ZFIN Image: Anelli et al., 2017, Fig. 3 S1

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Figure Caption

Fig. 3 S1

Gene expression changes induced by BRAF^V600E in larval thyrocytes.

(A) qPCR analysis of select up-regulated genes; data are represented as fold change of tg-BRAF^V600E-TOM compared to tg-TOM and are normalized using gapdh as a control. (B) GSEA hallmark gene sets significantly enriched in tg-BRAF^V600E-TOM (top 10 signatures, FDR < 25%, nominal p-value<5%). (C) GSEA enrichment plot of ERK signature using RNAseq data generated from sorted zebrafish thyrocytes as described. (D) Heat-map of ERK signature genes (n = 37) differentially expressed in tg-BRAF^V600E-TOM sorted thyrocytes compared to tg-TOM. (E) Validation of differential expression of select ERK signature genes using qPCR, data are normalized using gapdh as a control. (F) E-cadherin immunostaining in tg-TOM and tg-BRAF^V600E-TOM larvae at 5dpf.

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Acknowledgments

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