ZFIN Figure: Monteiro et al., 2016, Fig. 3

Fig. 3

TGFβ1 and TGFβ3 Are Required for Specification of HSCs

(A) Expression of runx1 in wild-type, tgfb1, tgfb2, and tgfb3 morphants.

(B) Expression of kdrl in wild-type, tgfb1, tgfb2, and tgfb3 morphants.

(C) Expression of dll4 in wild-type, tgfb1, tgfb2, and tgfb3 morphants.

(D) Expression of dlC in wild-type, tgfb1, tgfb2, and tgfb3 morphants. All samples were analyzed at 28 hpf.

(E-G) Maximum projections of itga2b:GFP; Kdrl:HRas-mCherry transgenic embryos in (E) uninjected, (F) tgfb1 morphants, and (G) tgfb3 morphants at 48 hpf. The images show part of the trunk DA and white arrowheads denote itga2b:GFP⁺ (green), kdrl:HRas-mCherry⁺ (magenta) HSPCs.

(H) HSPCs counts in the entire trunk region of uninjected, tgfb1, and tgfb3 morphant itga2b:GFP; Kdrl:HRas-mCherry transgenic embryos at 48 hpf (p value is indicated on the graph, n = 10 for each of the conditions).

(I-K) Expression of rag1 in the thymus at 4 dpf (red arrowheads) in (I) wild-type, (J) tgfb1 morphants, and (K) tgfb3 morphants.

(L) Expression of efnB2a in wild-type, tgfb1, and tgfb3 morphants.

(M) Expression of gata2b in wild-type, tgfb1, and tgfb3 morphants. The numbers of embryos are shown in each panel as the number of embryos with phenotype/total number analyzed.

See also Figure S3.

Expression Data

Genes:	dlc dll4 efnb2a EGFP gata2b kdrl mCherry rag1 runx1
Fish:	WT WT + MO2-tgfb3 WT + MO3-tgfb2 la2Tg; s843Tg la2Tg; s843Tg + MO2-tgfb3 WT + MO2-tgfb1b + MO5-tgfb1a la2Tg; s843Tg + MO2-tgfb1b + MO5-tgfb1a
Knockdown Reagents:	MO2-tgfb1b MO2-tgfb3 MO3-tgfb2 MO5-tgfb1a
Anatomical Terms:	artery artery vascular endothelium hematopoietic multipotent progenitor cell thymus T cell vascular endothelium ventral wall of dorsal aorta ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-tgfb3 la2Tg; s843Tg + MO2-tgfb3 WT + MO2-tgfb1b + MO5-tgfb1a la2Tg; s843Tg + MO2-tgfb1b + MO5-tgfb1a
Knockdown Reagents:	MO2-tgfb1b MO2-tgfb3 MO5-tgfb1a
Observed In:	thymus T cell ventral wall of dorsal aorta ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 38(4), Monteiro, R., Pinheiro, P., Joseph, N., Peterkin, T., Koth, J., Repapi, E., Bonkhofer, F., Kirmizitas, A., Patient, R., Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells, 358-70, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell