FIGURE

Fig. 6

ID

ZDB-FIG-160926-27

Publication

Monteiro et al., 2016 - Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells

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Fig. 6

Jag1a Is Required Downstream of TGF² Signaling for HSC Specification

(A) Expression of arterial markers in jag1a^MO is unaffected when compared to wild-type embryos.

(B) Expression of runx1, cmyb, and gfi1aa in wild-type and jag1a morphants (MO) at 28 hpf. All the markers analyzed are reduced or absent in jag1a morphants. Red arrowheads indicate the remaining gene expression in the floor of the DA of jag1a morphants.

(C) HSPCs (yellow arrowheads) are severely reduced in the CHT of itga2b:GFP;Kdrl:HsRas-mCherry transgenic embryos at 48 hpf injected with the jag1a^MO. Itga2b:GFP⁺ cells, magenta; Kdrl-HsRas:mCherry⁺ cells, green.

(D) Overexpression of jag1a with a Kdrl:jag1-V5 construct partially rescues the loss of runx1 and cmyb in the floor of the DA. 15 pg of the construct was used for this experiment. The numbers of embryos are shown in each panel as the number of embryos with phenotype/total number analyzed.

(E) Quantitation of the rescue effect observed in (D).

See also Figure S6.

Expression Data

Genes:	EGFP gfi1aa hey2 mCherry myb notch3 runx1
Fish:	WT WT + MO1-tgfbr2b WT + MO2-jag1a la2Tg; s843Tg la2Tg; s843Tg + MO2-jag1a
Knockdown Reagents:	MO1-tgfbr2b MO2-jag1a
Anatomical Terms:	artery trunk vasculature ventral wall of dorsal aorta ventral wall of dorsal aorta hematopoietic multipotent progenitor cell
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tgfbr2b WT + MO2-jag1a la2Tg; s843Tg + MO2-jag1a
Knockdown Reagents:	MO1-tgfbr2b MO2-jag1a
Observed In:	hematopoietic multipotent progenitor cell intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell ventral wall of dorsal aorta
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 38(4), Monteiro, R., Pinheiro, P., Joseph, N., Peterkin, T., Koth, J., Repapi, E., Bonkhofer, F., Kirmizitas, A., Patient, R., Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells, 358-70, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell