ZFIN ID: ZDB-FIG-160818-31
Muthu et al., 2016 - Rx3 and Shh direct anisotropic growth and specification in the zebrafish tuberal/anterior hypothalamus. Development (Cambridge, England)   143(14):2651-63 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

Rx3 suppresses dorsal and ventro-tuberal progenitors. (A-I) Side views of control embryos or embryos in which rx3 is absent. A′,B′,F′ show high-power views of boxed regions in A,B,F. Blue arrowheads and red arrows in A-D point to nkx2.1+ cells, which are absent in chk mutants. Blue arrows in C,D point to nkx2.1+ ventral-tuberal domain. Red arrowheads in F point to ectopic pax6+ cells. Black dotted lines indicate outline of ventral hypothalamus. Red dotted lines as in Fig. 2. Purple arrowhead in G′ points to rx3+pea3+ cells. H,I show views of isolated neuroectoderm. (J,K) Schematics of expression patterns in chk sibling (J) or mutant (K) 55hpf embryos. White and red dotted lines as in Fig. 2 AH, anterior hypothalamus; PH, posterior hypothalamus; PT, posterior tuberculum; Tel, telencephalon; (c)(r)Tub, (caudal) (rostral) tuberal hypothalamus. Scale bars: 50┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv4 WT standard conditions Prim-15 hypothalamus ISH
Long-pec Not Detected hypothalamus ISH
WT + MO1-rx3 + MO3-rx3 standard conditions Long-pec hypothalamus ISH
nkx2.1 WT standard conditions Prim-5 caudal hypothalamic zone ISH
Prim-5 hypothalamus ISH
Prim-5 hypothalamus anterior region ISH
Prim-5 telencephalon ISH
Long-pec caudal tuberculum ISH
Long-pec hypothalamus ISH
Long-pec hypothalamus anterior region ISH
Long-pec hypothalamus posterior region ISH
Long-pec telencephalon ISH
rx3w29/w29 standard conditions Prim-5 Not Detected caudal hypothalamic zone ISH
Prim-5 hypothalamus ISH
Prim-5 Not Detected hypothalamus anterior region ISH
Prim-5 telencephalon ISH
Long-pec caudal hypothalamic zone ISH
Long-pec Not Detected caudal hypothalamic zone anterior region ISH
Long-pec caudal tuberculum ISH
Long-pec hypothalamus anterior region ISH
Long-pec hypothalamus posterior region ISH
pax6a WT standard conditions Long-pec hypothalamus dorsal region ISH
Long-pec thalamus ISH
rx3w29/w29 standard conditions Long-pec caudal hypothalamic zone anterior region ISH
Long-pec hypothalamus dorsal region ISH
Long-pec thalamus ISH
rx3 WT standard conditions Prim-15 hypothalamus ISH
Long-pec hypothalamus ISH
rx3w29/w29 standard conditions Long-pec hypothalamus ISH
shha WT standard conditions Long-pec hypothalamus ISH
rx3w29/w29 standard conditions Long-pec hypothalamus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rx3 + MO3-rx3 standard conditions Long-pec hypothalamus etv4 expression increased amount, abnormal
rx3w29/w29 standard conditions Prim-5 caudal hypothalamic zone nkx2.1 expression absent, abnormal
Prim-5 hypothalamus anterior region nkx2.1 expression absent, abnormal
Long-pec caudal hypothalamic zone anterior region nkx2.1 expression absent, abnormal
Long-pec caudal hypothalamic zone anterior region pax6a expression mislocalised, abnormal
Long-pec hypothalamus rx3 expression spatial pattern, abnormal
Long-pec hypothalamus shha expression decreased amount, abnormal
Long-pec hypothalamus anterior region nkx2.1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development