ZFIN ID: ZDB-PUB-160619-3
Rx3 and Shh direct anisotropic growth and specification in the zebrafish tuberal/anterior hypothalamus
Muthu, V., Eachus, H., Ellis, P., Brown, S., Placzek, M.
Date: 2016
Source: Development (Cambridge, England)   143(14): 2651-63 (Journal)
Registered Authors:
Keywords: Hypothalamus development, Anterior hypothalamus, Rx3, Sonic hedgehog, Tuberal hypothalamus, Zebrafish hypothalamus
MeSH Terms:
  • Animals
  • Anisotropy
  • Body Patterning*
  • Cell Differentiation
  • Cell Proliferation
  • Cell Survival
  • Hedgehog Proteins/metabolism*
  • Homeodomain Proteins/metabolism*
  • Hypothalamus, Anterior/growth & development*
  • Hypothalamus, Anterior/metabolism*
  • Neurons/cytology
  • Neurons/metabolism
  • Signal Transduction
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed: 27317806 Full text @ Development
In the developing brain, growth and differentiation are intimately linked. Here we show that in the zebrafish embryo, the homeodomain transcription factor rx3 co-ordinates these processes to build the tuberal/anterior hypothalamus. Analysis of rx3 chk mutant/rx3 morphant fish and EdU pulse-chase studies reveal that rx3 is required to select tuberal/anterior hypothalamic progenitors and to orchestrate their anisotropic growth. In the absence of rx3 function, progenitors accumulate in the 3(rd) ventricular wall, die or are inappropriately-specified, the shh+ anterior recess does not form, and its resident pomc+, ff1b+ and otp+ TH+ cells fail to differentiate. Manipulation of Shh signalling shows that shh co-ordinates progenitor cell selection and behaviour by acting as an on-off switch for rx3 Together our studies show that shh and rx3 govern formation of a distinct progenitor domain that elaborates pattern through its anisotropic growth and differentiation.