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ZFIN ID: ZDB-FIG-160816-2
Venero Galanternik et al., 2015 - Heparan Sulfate Proteoglycans Regulate Fgf Signaling and Cell Polarity during Collective Cell Migration. Cell Reports   10(30):414-428 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 2

The Wnt/β-Catenin Pathway Is Ectopically Activated in extl3/ext2 Mutants

(A-M′) In extl3/ext2 mutants Wnt/β-catenin target genes expand progressively between 34 and 48 hpf.

(F, H, J, and L) Sibling prim migration has finished by 48 hpf.

(G, I, K, and M) In extl3/ext2 mutants, the prim stalls and lef1, axin2, sef, and fgf10 are upregulated.

(G′, I′, K′, and M′) Magnified views of extl3/ext2 mutant prim. Black arrows: Wnt/β-catenin target expression in interneuromast cells.

(N-U′) Time course of the Wnt/β-catenin target wnt10a and inhibitor dkk1b. Upregulation of wnt10a correlates with the downregulation of dkk1b.

(O, O′, S, and S′) Mild downregulation of dkk1b corresponds with the expansion of wnt10a at 40-42 hpf.

(P, P′, T, and T′) By 44-46 hpf, wnt10a expands along the prim and dkk1b expression is almost lost.

(Q, Q′, U, and U′) By 48-50 hpf, dkk1b expression is entirely reduced, resulting in complete expansion of wnt10a.

(V-Y′′) The Fgf target pea3 is gradually lost in the prim (V-Y′) but is progressively activated in surrounding cells (V′′-Y′′).

(Z and Z′) Lower-magnification images of the 44-46 hpf embryos in X, X′, and X′′.

See also Figures S2 and S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
dkk1b WT standard conditions Prim-15 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium ISH
High-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium ISH
High-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
etv4 WT standard conditions Prim-15 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium ISH
High-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Prim-15 posterior lateral line primordium anatomical region ISH
Prim-25 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium anatomical region ISH
High-pec posterior lateral line primordium ISH
High-pec posterior lateral line primordium anatomical region ISH
Long-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium anatomical region ISH
fgf3 WT standard conditions Prim-15 posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
fgf10a WT standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
il17rd WT standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
lef1 WT standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
wnt10a WT standard conditions Prim-15 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium ISH
High-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium ISH
Prim-25 posterior lateral line primordium ISH
High-pec posterior lateral line primordium ISH
Long-pec posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ext2to79c/to79c ; extl3tm70g/tm70g standard conditions Prim-15 posterior lateral line primordium etv4 expression decreased distribution, abnormal
Prim-15 posterior lateral line primordium lef1 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium axin2 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium fgf10a expression increased distribution, abnormal
Prim-15 posterior lateral line primordium il17rd expression increased distribution, abnormal
Prim-15 posterior lateral line primordium fgf3 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium anatomical region etv4 expression increased distribution, abnormal
Prim-25 posterior lateral line primordium etv4 expression decreased distribution, abnormal
Prim-25 posterior lateral line primordium dkk1b expression decreased distribution, abnormal
Prim-25 posterior lateral line primordium wnt10a expression increased distribution, abnormal
Prim-25 posterior lateral line primordium anatomical region etv4 expression increased distribution, abnormal
High-pec posterior lateral line primordium etv4 expression decreased distribution, abnormal
High-pec posterior lateral line primordium dkk1b expression decreased distribution, abnormal
High-pec posterior lateral line primordium wnt10a expression increased distribution, abnormal
High-pec posterior lateral line primordium anatomical region etv4 expression increased distribution, abnormal
Long-pec posterior lateral line primordium etv4 expression decreased distribution, abnormal
Long-pec posterior lateral line primordium dkk1b expression decreased distribution, abnormal
Long-pec posterior lateral line primordium il17rd expression increased distribution, abnormal
Long-pec posterior lateral line primordium wnt10a expression increased distribution, abnormal
Long-pec posterior lateral line primordium fgf10a expression increased distribution, abnormal
Long-pec posterior lateral line primordium lef1 expression increased distribution, abnormal
Long-pec posterior lateral line primordium axin2 expression increased distribution, abnormal
Long-pec posterior lateral line primordium anatomical region etv4 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.