FIGURE

Fig. 1

ID

ZDB-FIG-160816-1

Publication

Venero Galanternik et al., 2015 - Heparan Sulfate Proteoglycans Regulate Fgf Signaling and Cell Polarity during Collective Cell Migration

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Fig. 1

Loss of HSPGs Results in LL Prim Migration and Patterning Defects

(A, E, I, and M) gpc1b expression in (A) WT, (E) apc^mcr, (I) DMSO-treated, and (M) SU5402-treated embryos.

(B, C, F, G, J, K, N, and O) gpc4 and sdc3 expression in (B and C) WT, (F and G) apc^mcr, (J and K) DMSO-treated, and (N and O) SU5402-treated embryos.

(D, H, L, and P) sdc4 expression in (D) WT, (H) apc^mcr, (L) DMSO-treated, and (P) SU5402-treated embryos.

(G and H) Black arrowheads point to the ectopic halo of sdc3 and sdc4.

(Q and R) WT and extl3 mutant prim has reached the tail tip by 48 hpf.

(S and T) ext2 and extl3/ext2 prim fails to complete migration.

(Q′-T′) Magnification of prim at 46 hpf.

(Q′ and R′) WT and extl3 mutant prim shows a normal morphology.

(S′) ext2 mutant prim possesses a pointed tip (white arrow).

(T′) extl3/ext2 mutant prim shows severe morphological defects. The leading edge elongates (white arrow) and rosettes are closely spaced (yellow arrowheads).

(U-W) Still images of time-lapse movies: WT (U), ext2 (V), and extl3/ext2 mutant prim (W). Yellow arrows in (V) and (W) point to ectopic filopodia.

See also Figure S2 and Movies S1, S2, S3, and S4.

Expression Data

Genes:	EGFP gpc1b gpc4 sdc3 sdc4
Fish:	WT apc^hu745/hu745 ext2^to79c/to79c; zf106Tg extl3^tm70g/tm70g; zf106Tg ext2^to79c/to79c; extl3^tm70g/tm70g; zf106Tg
Condition:	chemical treatment by environment: SU5402
Anatomical Terms:	posterior lateral line primordium posterior lateral line primordium anatomical region
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT apc^hu745/hu745 ext2^to79c/to79c; zf106Tg extl3^tm70g/tm70g; zf106Tg ext2^to79c/to79c; extl3^tm70g/tm70g; zf106Tg
Condition:	chemical treatment by environment: SU5402
Observed In:	interneuromast cell interneuromast cell cell projection neuromast neuromast cell projection posterior lateral line neuromast posterior lateral line neuromast deposition posterior lateral line neuromast primordium migration posterior lateral line primordium
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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