FIGURE

Fig. 5

ID

ZDB-FIG-160525-38

Publication

San et al., 2016 - Normal formation of a vertebrate body plan and loss of tissue maintenance in the absence of ezh2

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Fig. 5

Myocardial development is affected in MZezh2 embryos.

(a) In situ hybridization for different heart markers in MZezh2^hu5670/+ and MZezh2^{hu5670/hu5670} at various time points of development. hand2 is an early myocardial marker. myh6 is a marker for atrial cells. vmhc is a marker for ventricular cells. nppa is a late myocardial marker. nfat-c1 is an endocardial marker. All these markers are expressed in MZezh2^{hu5670/hu5670}, although vmhc, nppa, and nfat-c1 expression show a smaller number of positive cells. (b) In situ hybridization for nkx2.5 at different time points after fertilization in MZezh2^hu5670/+ and MZezh2^{hu5670/hu5670} embryos. Arrow heads point to cells of the pharyngeal arch artery progenitors. This is absent in MZezh2^{hu5670/hu5670}. (c) In situ hybridization for has2 and fgf24 at 2 dpf in MZezh2^{hu5670/hu5670} and their heterozygous siblings. In MZezh2^hu5670/+ expression is restricted to the heart (arrow heads), whereas in the MZezh2^{hu5670/hu5670} embryos expression is visible in the area surrounding the heart tube (encircled by dashed line). For fgf24 this is also shown from a lateral view (arrow heads). Scale bar is 200 µm. The numbers indicate the number of embryos with the displayed phenotype compared to the total number of embryos analyzed.

Expression Data

Genes:	fgf24 hand2 has2 myh6 myh7 nfatc1 nkx2.5 nppa
Fish:	ezh2^{hu5670/hu5670} ezh2^hu5670/+
Anatomical Terms:	atrioventricular canal atrioventricular canal anatomical region endocardium heart heart anatomical region heart primordium heart tube myocardium pharyngeal arch artery presumptive atrium heart tube presumptive cardiac ventricle heart tube
Stage Range:	10-13 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ezh2^{hu5670/hu5670}
Observed In:	atrioventricular canal anatomical region cardiac ventricle endocardium heart heart anatomical region heart development heart looping myocardium pharyngeal arch artery presumptive cardiac ventricle heart tube
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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