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ZFIN ID: ZDB-FIG-160525-39
San et al., 2016 - Normal formation of a vertebrate body plan and loss of tissue maintenance in the absence of ezh2. Scientific Reports   6:24658 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

MZezh2 mutant embryos display impaired myocardial and gastrointestinal tissue maintenance.

(a) In situ hybridization for myl7 at different time points in MZezh2hu5670/+ and MZezh2hu5670/hu5670. At 1 dpf the heart of MZezh2hu5670/hu5670 is straight. In MZezh2hu5670/hu5670 embryos myl7 expressing cells are found outside the heart at 1.5 dpf (arrow heads). At 2 dpf the number of myl7 expressing cells is decreased in MZezh2hu5670/hu5670 compared to MZezh2hu5670/+. (b) Stills of time lapse (Supplementary Movie S1, 2) imaging of Tg(myl7::GFP) MZezh2hu5670/+ and MZezh2hu5670/hu5670 embryos from 1 to 2 dpf. In MZezh2hu5670/hu5670 embryos, GFP-positive cells are moving away from the heart (arrows). Arrow heads point at ventricle and atrium. (c) Immunostaining for GFP to visualize Tg(myl7::GFP) (brown precipitation, arrow heads) combined with in situ hybridization for nkx2.5 (purple staining, arrows) at 2 dpf. In MZezh2hu5670/+ no nkx2.5 expressing cells are present. In MZezh2hu5670/hu5670 cells that are detached from the heart express nkx2.5. The right panel shows a zoom-in of the left panel (white square). Scale bar is 50 µm. (d) Immunostaining for GFP Tg(myl7::GFP) combined with in situ hybridization for nppa at 2 dpf in MZezh2hu5670/+ and MZezh2hu5670/hu5670 embryos. Two embryos per genotype are shown. In MZezh2hu5670/hu5670 embryos nppa expression is absent in one and not ubiquitous in the other embryo (arrow heads). In MZezh2hu5670/+ nppa is expressed in atrium and ventricle (arrow heads). Scale bar is 50 µm. (e) In situ hybridization for different gastrointestinal tract markers at 1 and 2 dpf in MZezh2hu5670/hu5670 and MZezh2hu5670/+. Expression of gata6 is present in both MZezh2hu5670/hu5670 and MZezh2hu5670/+ at 1 and 2 dpf. At 2 dpf the intestinal tube appears straight in the MZezh2hu5670/hu5670, whereas structures like the liver and pancreas can be seen in MZezh2hu5670/+ (arrow heads). MZezh2hu5670/hu5670 are able to form a gastrointestinal tract, observed by in situ hybridization for foxa3 at 2 dpf, although the organs are bilaterally formed (arrow heads). In MZezh2hu5670/hu5670 no expression of terminal differentiation markers for liver, fabp10, and exocrine pancreas, try, was observed. Scale bar is 100 µm. Numbers indicate the number of embryos with the displayed phenotype compared to the total number of embryos analyzed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ezh2hu5670/+ ; twu34Tg standard conditions Prim-15 presumptive atrium heart tube IFL
Prim-15 presumptive cardiac ventricle heart tube IFL
Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IHC
twu34Tg ; ezh2hu5670/hu5670 standard conditions Prim-15 presumptive atrium heart tube IFL
Prim-15 presumptive atrium heart tube anatomical region IFL
Prim-15 presumptive cardiac ventricle heart tube IFL
Prim-15 presumptive cardiac ventricle heart tube anatomical region IFL
Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Long-pec heart IHC
Long-pec heart anatomical region IHC
fabp10a ezh2hu5670/+ standard conditions Long-pec liver ISH
ezh2hu5670/hu5670 standard conditions Long-pec Not Detected liver ISH
foxa3 ezh2hu5670/+ standard conditions Long-pec endoderm ISH
Long-pec gut ISH
ezh2hu5670/hu5670 standard conditions Long-pec endoderm ISH
Long-pec gut ISH
gata6 ezh2hu5670/+ standard conditions Prim-5 endoderm ISH
Long-pec gut ISH
ezh2hu5670/hu5670 standard conditions Prim-5 endoderm ISH
Long-pec gut ISH
myl7 ezh2hu5670/+ standard conditions 10-13 somites myocardial precursor ISH
Prim-5 heart tube ISH
Prim-25 heart tube ISH
Long-pec heart ISH
ezh2hu5670/hu5670 standard conditions 10-13 somites myocardial precursor ISH
Prim-5 heart tube ISH
Prim-5 heart tube anatomical region ISH
Prim-25 heart tube anatomical region ISH
Long-pec heart ISH
nkx2.5 ezh2hu5670/+ ; twu34Tg standard conditions Long-pec heart ISH
twu34Tg ; ezh2hu5670/hu5670 standard conditions Long-pec heart ISH
Long-pec heart anatomical region ISH
nppa ezh2hu5670/+ ; twu34Tg standard conditions Long-pec heart ISH
twu34Tg ; ezh2hu5670/hu5670 standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
prss1 ezh2hu5670/+ standard conditions Long-pec exocrine pancreas ISH
ezh2hu5670/hu5670 standard conditions Long-pec Not Detected exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ezh2hu5670/hu5670 standard conditions Prim-5 heart straight, abnormal
Prim-5 heart tube anatomical region myl7 expression mislocalised, abnormal
Prim-25 cardiac muscle cell detached from atrium, abnormal
Prim-25 cardiac muscle cell detached from cardiac ventricle, abnormal
Prim-25 heart decreased size, abnormal
Prim-25 heart morphology, abnormal
Prim-25 heart wholeness, abnormal
Prim-25 heart development process quality, abnormal
Prim-25 heart jogging decreased occurrence, abnormal
Prim-25 heart looping decreased occurrence, abnormal
Prim-25 heart tube anatomical region myl7 expression mislocalised, abnormal
Long-pec embryonic digestive tract morphogenesis process quality, abnormal
Long-pec exocrine pancreas absent, abnormal
Long-pec exocrine pancreas prss1 expression absent, abnormal
Long-pec gut morphology, abnormal
Long-pec gut straight, abnormal
Long-pec heart myl7 expression decreased distribution, abnormal
Long-pec heart decreased size, abnormal
Long-pec heart morphology, abnormal
Long-pec heart development process quality, abnormal
Long-pec heart jogging decreased occurrence, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec liver absent, abnormal
Long-pec liver fabp10a expression absent, abnormal
twu34Tg ; ezh2hu5670/hu5670 standard conditions Prim-15 cardiac muscle cell detached from atrium, abnormal
Prim-15 cardiac muscle cell detached from cardiac ventricle, abnormal
Prim-15 heart decreased size, abnormal
Prim-15 heart wholeness, abnormal
Prim-15 heart development process quality, abnormal
Prim-15 heart jogging decreased occurrence, abnormal
Prim-15 heart looping decreased occurrence, abnormal
Prim-15 presumptive atrium heart tube anatomical region EGFP expression mislocalised, abnormal
Prim-15 presumptive cardiac ventricle heart tube anatomical region EGFP expression mislocalised, abnormal
Long-pec atrium nppa expression decreased amount, abnormal
Long-pec cardiac ventricle nppa expression decreased amount, abnormal
Long-pec heart decreased size, abnormal
Long-pec heart anatomical region EGFP expression mislocalised, abnormal
Long-pec heart anatomical region nkx2.5 expression mislocalised, abnormal
Long-pec heart development process quality, abnormal
Long-pec heart jogging decreased occurrence, abnormal
Long-pec heart looping decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.