ZFIN Image: San et al., 2016, Fig. 5

Image

Figure Caption

Fig. 5

Myocardial development is affected in MZezh2 embryos.

(a) In situ hybridization for different heart markers in MZezh2^hu5670/+ and MZezh2^{hu5670/hu5670} at various time points of development. hand2 is an early myocardial marker. myh6 is a marker for atrial cells. vmhc is a marker for ventricular cells. nppa is a late myocardial marker. nfat-c1 is an endocardial marker. All these markers are expressed in MZezh2^{hu5670/hu5670}, although vmhc, nppa, and nfat-c1 expression show a smaller number of positive cells. (b) In situ hybridization for nkx2.5 at different time points after fertilization in MZezh2^hu5670/+ and MZezh2^{hu5670/hu5670} embryos. Arrow heads point to cells of the pharyngeal arch artery progenitors. This is absent in MZezh2^{hu5670/hu5670}. (c) In situ hybridization for has2 and fgf24 at 2 dpf in MZezh2^{hu5670/hu5670} and their heterozygous siblings. In MZezh2^hu5670/+ expression is restricted to the heart (arrow heads), whereas in the MZezh2^{hu5670/hu5670} embryos expression is visible in the area surrounding the heart tube (encircled by dashed line). For fgf24 this is also shown from a lateral view (arrow heads). Scale bar is 200 µm. The numbers indicate the number of embryos with the displayed phenotype compared to the total number of embryos analyzed.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.