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ZFIN ID: ZDB-FIG-150910-31
Sesé et al., 2013 - SMYD2 is induced during cell differentiation and participates in early development. The International journal of developmental biology   57(5):357-364 Full text @ Int. J. Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Sphere to Bud

Fig. 5

Effects of smyd2a knockdown on gene expression during zebrafish gastrulation. (A) In situ hybridization of ntl in Control-MO and smyd2a-MO injected embryos at 6 hpf. Embryos are shown in lateral views (left), and animal pole views (right). (B) mRNA levels of indicated genes in Control-MO and smyd2a-MO embryos at 4, 6, 8, and 10 hpf. 50 embryos were collected at each point. Mean and standard deviation of triplicate quantifications are shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp2a AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
chrd AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
ctnnb1 AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
gsc AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
mixl1 AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
sox32 AB control Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
AB + MO4-smyd2a standard conditions Sphere whole organism RTPCR
Shield whole organism RTPCR
75%-epiboly whole organism RTPCR
Bud whole organism RTPCR
tbxta AB control Shield margin ISH
AB + MO4-smyd2a standard conditions Shield Not Detected margin ISH
Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal The International journal of developmental biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Dev. Biol.