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ZFIN ID: ZDB-FIG-150528-2
Liu et al., 2015 - DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish. Journal of Hematology & Oncology   8:15 Full text @ J. Hematol. Oncol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Day 5

Fig. 1

Impairment of definitive hematopoiesis in dnmt1 mutant zebrafish. (A-J) WISH analysis of cmyb expression from 36 hpf to 5 dpf. In ldd794 mutant, cmyb expression was decreased from 36 hpf (A-B) and absent at 5 dpf (I-J). (K, K′, L, L′) Expression of hematopoietic progenitor marker runx1 was decreased at 36 hpf. (K′, L′) Magnified images of the boxed regions in K and L, respectively. (M-N) Expression of hematopoietic progenitor marker scl was decreased at 5 dpf. (O-Z) WISH analysis of key hematopoietic markers in dnmt1 mutant embryos and wild-type siblings. Expression of erythrocyte progenitor marker gata1 (O-P), mature erythrocyte marker hbae1(Q, R), myeloid-specific marker mpx (S, T), macrophage marker lyz (U, V), l-plastin (W, X) were decreased. Expression of lymphocyte maker rag1 (Y, Y′, Z, Z′) was completely absent at 4 dpf. Blue arrows indicate the position of the thymus; red arrows indicate the CHT; black arrows indicate the AGM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a TU control Day 5 nucleate erythrocyte ISH
dnmt1rj794/rj794(TU) control Day 5 nucleate erythrocyte ISH
hbae1.1 TU control Day 5 nucleate erythrocyte ISH
dnmt1rj794/rj794(TU) control Day 5 nucleate erythrocyte ISH
lcp1 TU control Day 5 myeloid cell ISH
dnmt1rj794/rj794(TU) control Day 5 myeloid cell ISH
lyz TU control Day 5 myeloid cell ISH
dnmt1rj794/rj794(TU) control Day 5 myeloid cell ISH
mpx TU control Prim-25 myeloid cell ISH
dnmt1rj794/rj794(TU) control Prim-25 myeloid cell ISH
myb TU control Prim-25 hematopoietic stem cell ISH
Long-pec hematopoietic stem cell ISH
Protruding-mouth hematopoietic stem cell ISH
Day 4 hematopoietic stem cell ISH
Day 5 hematopoietic stem cell ISH
dnmt1rj794/rj794(TU) control Prim-25 hematopoietic stem cell ISH
Long-pec hematopoietic stem cell ISH
Protruding-mouth hematopoietic stem cell ISH
Day 4 hematopoietic stem cell ISH
Day 5 hematopoietic stem cell ISH
rag1 TU control Day 4 thymus lymphocyte ISH
dnmt1rj794/rj794(TU) control Day 4 Not Detected thymus lymphocyte ISH
runx1 TU control Prim-25 hematopoietic stem cell ISH
dnmt1rj794/rj794(TU) control Prim-25 hematopoietic stem cell ISH
tal1 TU control Day 5 hematopoietic stem cell ISH
dnmt1rj794/rj794(TU) control Day 5 hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1rj794/rj794(TU) control Prim-25 definitive hemopoiesis process quality, abnormal
dnmt1rj794/rj794(TU) control Prim-25 hematopoietic stem cell myb expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Prim-25 hematopoietic stem cell runx1 expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Prim-25 myeloid cell mpx expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Long-pec definitive hemopoiesis process quality, abnormal
dnmt1rj794/rj794(TU) control Long-pec hematopoietic stem cell myb expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Protruding-mouth definitive hemopoiesis process quality, abnormal
dnmt1rj794/rj794(TU) control Protruding-mouth hematopoietic stem cell myb expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 4 definitive hemopoiesis process quality, abnormal
dnmt1rj794/rj794(TU) control Day 4 hematopoietic stem cell myb expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 4 thymus lymphocyte rag1 expression absent, abnormal
dnmt1rj794/rj794(TU) control Day 5 definitive hemopoiesis process quality, abnormal
dnmt1rj794/rj794(TU) control Day 5 hematopoietic stem cell tal1 expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 5 hematopoietic stem cell myb expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 5 myeloid cell lcp1 expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 5 myeloid cell lyz expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 5 nucleate erythrocyte hbae1.1 expression decreased distribution, abnormal
dnmt1rj794/rj794(TU) control Day 5 nucleate erythrocyte gata1a expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Hematology & Oncology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Hematol. Oncol.