FIGURE

Fig. 4

ID

ZDB-FIG-150528-4

Publication

Liu et al., 2015 - DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish

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Fig. 4

Fluorescent images of the Tg(cmyb-EGFP) line, Q-PCR result of cebpa expression, and bisulfite sequencing PCR (BSP) assay. (A) Fluorescent images of the Tg (cmyb-EGFP) line at 3 dpf. EGFP positive cells in Tg (cmyb:EGFP) dnmt1 morphants were sharply decreased compared with wild-type ones. (B) Cebpa expression level in wild-type and dnmt1 knockdown cmyb-EGFP positive cells. Q-PCR result indicated that there was a significant increase of cebpa expression level in dnmt1 morphants compared with wild-type ones. (C) BSP analysis of putative cebpa regulation regions in cmyb-EGFP positive cells at 3 dpf. The methylation levels of cebpa regulation regions in dnmt1 morphants were significantly decreased in all the four chosen regions compared to those in wild-type embryos.

Expression Data

Gene:	EGFP
Fish:	zf169Tg zf169Tg + MO4-dnmt1
Knockdown Reagent:	MO4-dnmt1
Anatomical Term:	caudal hematopoietic tissue
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf169Tg + MO4-dnmt1
Knockdown Reagent:	MO4-dnmt1
Observed In:	caudal hematopoietic tissue definitive hemopoiesis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.