ZFIN ID: ZDB-FIG-150129-26
Herrero-Turrión et al., 2014 - Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine: identification of new genes associated with neuronal function and mu opioid receptor expression. BMC Genomics   15:874 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Fig. 7

Knockdown of µ opioid receptor ( oprm1 ). Expression levels of grb2, acox1, dao.1, wls, grik-l, otpb, copb2, bnip4, camk1gb, sox19b, magi1b and plg of the Control group, the Morpholine (MO) control, Morpholine oprm1 (oprm1-MO) and oprm1-MO exposure to morphine (oprm1-MO + morphine). The expression levels were quantified using RT-qPCR analysis and were normalized to β-actin expression. Each bar represents the FC ± SD (n = 8). Data were analyzed by one-way ANOVA and using the Tukey post-hoc test. P values of < 0.05 being considered statistically significant (***p < 0.001; **p < 0.01; *p <0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acox1 AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
bnip4 AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
camk1gb AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
copb2 AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
dao.1 AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
grb2b AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
magi1b AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
otpb AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
plg AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
si:ch211-251b21.1 AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
sox19b AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
wls AB control Prim-5 whole organism RTPCR
AB + MO1-oprm1 chemical treatment: pharmaceutical Prim-5 whole organism RTPCR
AB + MO1-oprm1 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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