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ZFIN ID: ZDB-FIG-141230-22
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 90%-epiboly to Prim-5

Fig. 3 Expression analyses reveal that mesoderm development, but not induction, is disupted in spt;ntl double mutant embryos. In situ hybridization and immunohistochemistry in wild-type (A,E,I,M), ntl (B,F,J,N), spt (C,G,K,O) and spt;ntl (D,H,L,P) embryos reveal synergistic and partially redundant interaction of spt and ntl during mesoderm formation. (A-D) ntl expression at mid-gastrulation (75% epiboly, 8 hpf) in wild-type and mutant embryos. (E-H) her1 expression during late gastrulation (95% epiboly, 9.5 hpf) in wild-type and mutant presomitic mesoderm. In I-L, pax2.1, krox20, and myoD transcripts are visualized in blue and Ntl protein is visualized in brown. In 10-somite stage (14 hpf) wild-type embryos (I), pax2.1 is expressed in the mid-hindbrain boundary, the otic placode and the developing pronephros (black asterisks), krox20 in hindbrain rhombomeres 3 and 5 (white asterisks), myoD in adaxial cells (arrowhead) and a subset of cells within each formed somite and the two most anterior forming somites, and Ntl in developing notochord cells. As discussed in the text, mesodermal gene expression is variably disrupted in ntl and spt embryos (J,K) and is abolished in spt;ntl embryos (L). (M-P) tropomyosin expression at 24 hpf. Embryos with intact yolks (A-H) are dorsal views, with anterior to the top. Dorsal (I-L) and lateral (M-P) views of deyolked embryos are shown with anterior to the left. Scale bars: 100 μm in A for A-D; E for E-H; I for I-L and M for M-P .

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
tbx16b104/b104 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
tbxtab195/b195 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
her1 WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
tbx16b104/b104 standard conditions 90%-epiboly Not Detected whole organism ISH
tbxtab195/b195 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 90%-epiboly Not Detected whole organism ISH
myod1 WT standard conditions 10-13 somites adaxial cell ISH
10-13 somites somite ISH
tbx16b104/b104 standard conditions 10-13 somites segmental plate ISH
tbxtab195/b195 standard conditions 10-13 somites adaxial cell ISH
10-13 somites somite ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 10-13 somites Not Detected adaxial cell ISH
10-13 somites Not Detected somite ISH
pax2a WT standard conditions 10-13 somites midbrain hindbrain boundary neural keel ISH
10-13 somites otic placode ISH
10-13 somites pronephros ISH
tbx16b104/b104 standard conditions 10-13 somites midbrain hindbrain boundary neural keel ISH
10-13 somites otic placode ISH
10-13 somites pronephros ISH
tbxtab195/b195 standard conditions 10-13 somites midbrain hindbrain boundary neural keel ISH
10-13 somites otic placode ISH
10-13 somites pronephros ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 10-13 somites midbrain hindbrain boundary neural keel ISH
10-13 somites otic placode ISH
10-13 somites pronephros ISH
tbxta WT standard conditions 75%-epiboly margin ISH
Ab1-ta 10-13 somites notochord IHC
tbx16b104/b104 standard conditions 75%-epiboly margin ISH
Ab1-ta 10-13 somites notochord IHC
tbxtab195/b195 standard conditions 75%-epiboly margin ISH
Ab1-ta 10-13 somites Not Detected notochord IHC
tbxtab195/b195; tbx16b104/b104 standard conditions 75%-epiboly margin ISH
Ab1-ta 10-13 somites Not Detected notochord IHC
tpma WT standard conditions Prim-5 trunk musculature ISH
tbx16b104/b104 standard conditions Prim-5 trunk musculature ISH
tbxtab195/b195 standard conditions Prim-5 trunk musculature ISH
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 Not Detected trunk musculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions 10-13 somites mesoderm development process quality, abnormal
tbxtab195/b195 standard conditions 10-13 somites mesoderm development process quality, abnormal
tbxtab195/b195; tbx16b104/b104 standard conditions 90%-epiboly mesoderm development disrupted, abnormal
tbxtab195/b195; tbx16b104/b104 standard conditions 10-13 somites mesoderm development disrupted, abnormal
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 trunk musculature posterior region absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.