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ZIRC
ZFIN ID: ZDB-FIG-141230-24
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5 spt and ntl together are required for spinal cord medial floor plate formation. Expression of sonic hedgehog (shh) in ventral brain, medial floor plate (MFP), and posterior notochord and of krox20 in wild-type (A) and mutant (B-D) embryos at 24 hpf. Posterior MFP irregularities, such as broadening, are noted in ntl (B) and spt (C) embryos, but MFP is conspiciously missing in spt;ntl embryos (D), beginning approximately at the end of the hindbrain. Lateral views, with anterior to the left. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
shha WT standard conditions Prim-5 brain ventral region ISH
Prim-5 medial floor plate ISH
Prim-5 notochord ISH
tbx16b104/b104 standard conditions Prim-5 brain ventral region ISH
Prim-5 medial floor plate ISH
Prim-5 notochord ISH
tbxtab195/b195 standard conditions Prim-5 brain ventral region ISH
Prim-5 medial floor plate ISH
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 medial floor plate anterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions Prim-5 medial floor plate increased width, abnormal
tbxtab195/b195 standard conditions Prim-5 medial floor plate increased width, abnormal
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 floor plate development disrupted, abnormal
Prim-5 medial floor plate truncated, abnormal
Prim-5 medial floor plate posterior region absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.