header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-141230-21
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 2 spt and ntl are required together for trunk and tail mesoderm development. (A-D) Live wild-type and mutant embryos at 24 hpf. Embryos doubly homozygous for spt and ntl mutations (D) have more severe posterior mesodermal deficiencies than ntl (B) and spt (C) embryos. Anterior development appears unperturbed. (E-H) Tranverse sections through trunk regions of wild-type (E) and mutant embryos (F-H) at 30 hpf show that (F) ntl embryos lack a differentiated notochord, (G) spt embryos have reduced muscle tissue, and (H) spt;ntl embryos lack all recognizable mesoderm, having only a spinal cord covered by skin. sc, spinal cord; no, notochord; sm, somitic muscle; *, pronephric tubule. Scale bars, 250 μm (A), 25 μm (E).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions Prim-5 post-vent region spade-shaped, abnormal
Prim-15 whole organism posterior region has fewer parts of type muscle, abnormal
tbxtab195/b195 standard conditions Prim-5 post-vent region absent, abnormal
Prim-5 whole organism posterior region morphology, abnormal
Prim-15 notochord absent, abnormal
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 mesoderm development disrupted, abnormal
Prim-5 whole organism anterior region morphology, normal
Prim-5 whole organism posterior region lacks all parts of type mesoderm, abnormal
Prim-15 mesoderm development disrupted, abnormal
Prim-15 whole organism lacks all parts of type pronephros, abnormal
Prim-15 whole organism posterior region lacks all parts of type mesoderm, abnormal
Prim-15 whole organism posterior region morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.