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ZFIN ID: ZDB-FIG-141230-23
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 90%-epiboly to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 4 Neither spt nor ntl function is required for most dorsal-ventral spinal cord patterning. (A,E,I,M) Wild-type, (B,F,J,N) ntl, (C,G,K,O) spt, and (D,H,L,P) spt;ntl embryos. (A-D) pax2.1 interneuron expression at 24 hpf, (E-L) islet1 expression at the 8-10 somite stage (13-14 hpf); arrowheads indicate primary motoneurons and arrows indicate Rohon-Beard neurons. (M-P) sonic hedgehog (shh) and krox20 expression at the late gastrula stage (<9.5 hpf). Transverse sections (A-D; I-L) are approximately from midtrunk levels. Anterior is to the top. Scale bars: 25 μm (A-D and I-L), 100 μm (E-H and M-P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 90%-epiboly hindbrain neural plate ISH
tbx16b104/b104 standard conditions 90%-epiboly hindbrain neural plate ISH
tbxtab195/b195 standard conditions 90%-epiboly hindbrain neural plate ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 90%-epiboly hindbrain neural plate ISH
isl1 WT standard conditions 5-9 somites to 10-13 somites primary motor neuron ISH
5-9 somites to 10-13 somites Rohon-Beard neuron ISH
tbx16b104/b104 standard conditions 5-9 somites to 10-13 somites primary motor neuron ISH
5-9 somites to 10-13 somites Rohon-Beard neuron ISH
tbxtab195/b195 standard conditions 5-9 somites to 10-13 somites primary motor neuron ISH
5-9 somites to 10-13 somites Rohon-Beard neuron ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 5-9 somites to 10-13 somites primary motor neuron ISH
5-9 somites to 10-13 somites Rohon-Beard neuron ISH
pax2a WT standard conditions Prim-5 interneuron ISH
tbx16b104/b104 standard conditions Prim-5 interneuron ISH
tbxtab195/b195 standard conditions Prim-5 interneuron ISH
tbxtab195/b195; tbx16b104/b104 standard conditions Prim-5 interneuron ISH
shha WT standard conditions 90%-epiboly axis ISH
tbx16b104/b104 standard conditions 90%-epiboly axis ISH
tbxtab195/b195 standard conditions 90%-epiboly axis ISH
tbxtab195/b195; tbx16b104/b104 standard conditions 90%-epiboly axis anterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbxtab195/b195; tbx16b104/b104 standard conditions 5-9 somites - 10-13 somites primary motor neuron decreased amount, abnormal
5-9 somites - 10-13 somites primary motor neuron mislocalised, abnormal
Prim-5 interneuron decreased amount, abnormal
Prim-5 interneuron mislocalised medially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.