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ZFIN ID: ZDB-FIG-141230-20
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Shield to 5-9 somites

Fig. 1 Two zebrafish T-box genes, ntl and spt, are co-expressed in gastrula cells at the blastoderm margin. Spt protein expression in wild-type (A-E) and spt (F,G) embryos at midgastrula stage (70% epiboly) (D) and at the 6-somite stage (12 hpf) (A-C,E-F). (A) Spt is expressed strongly in adaxial (arrow) and tail bud (white asterisk) cells and more weakly in presomitic and lateral mesodermal cells (asterisk). (B) Double-labeling for Spt protein (brown) and ntl transcripts (blue); arrow indicates lateral presumptive pronephros. (C) Double-labeling for Spt protein (brown) and myoD transcripts (blue). Arrow indicates adaxial cells, black arrowhead indicates the posterior limit of myoD expression, and white arrowhead indicates the anterior extent of Spt protein expression. myoD is expressed in adaxial cells and in posterior cells of each formed somite (Weinberg et al., 1996). (D) Section through the lateral region of a midgastrula embryo (70% epiboly) double-labeled for Ntl (green) and Spt (orange) protein; white arrows indicate cells expressing both Ntl and Spt. (E) Sagittal section through the dorsal midline, double-labeled for Ntl (green) and Spt (orange); kv, Kuppfer’s vesicle. (F,G) Similar sections of spt embryos double-labeled for Ntl (F) and Spt (G). Scale bars: 50 μm in C for A-C and in G for D-G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tbx16 Ab2-tbx16 WT standard conditions Shield germ ring nucleus IHC
Ab2-tbx16 5-9 somites adaxial cell nucleus IHC
Ab2-tbx16 5-9 somites segmental plate nucleus IHC
Ab2-tbx16 5-9 somites tail bud nucleus IHC
Ab2-tbx16 tbx16b104/b104 standard conditions 5-9 somites Not Detected tail bud IHC
tbxta Ab1-ta WT standard conditions Shield germ ring IHC
Ab1-ta 5-9 somites tail bud IHC
Ab1-ta tbx16b104/b104 standard conditions 5-9 somites tail bud IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.