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ZIRC
ZFIN ID: ZDB-FIG-141230-25
Amacher et al., 2002 - The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. Development (Cambridge, England)   129(14):3311-3323
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 6 The nodal-related gene cyclops (cyc) is not required for medial floor plate development in the absence of spt function. sonic hedgehog (shh) expression in trunk medial floor plate (MFP) of wild-type (A) and mutant (B-D) embryos at 24 hpf. As noted already, spt MFP is often broader and some MFP cells are located ventral to the notochord (arrow) (C). In contrast, MFP is completely absent in the trunk of cyc embryos (B), but is significantly restored in spt;cyc embryos (D). Ventral brain shh expression in spt;cyc embryos is comparable to that in cyc embryos (data not shown); thus, suppression of the cyc MFP defect by the spt mutation is restricted to the trunk and tail. Lateral views, with anterior to the left. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
shha WT standard conditions Prim-5 medial floor plate ISH
ndr2Df(Chr12:hhex)b16/b16 standard conditions Prim-5 Not Detected medial floor plate ISH
tbx16b104/b104 standard conditions Prim-5 medial floor plate ISH
tbx16b104/b104; ndr2Df(Chr12:hhex)b16/b16 standard conditions Prim-5 medial floor plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ndr2Df(Chr12:hhex)b16/b16 standard conditions Prim-5 medial floor plate absent, abnormal
tbx16b104/b104 standard conditions Prim-5 medial floor plate increased width, abnormal
tbx16b104/b104 standard conditions Prim-5 medial floor plate cell mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.