ZFIN ID: ZDB-FIG-140626-11
Okigawa et al., 2014 - Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination. Developmental Biology   391(2):196-206 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. 4 Notch1a, Notch1b, and Notch3 together contribute to p2 progenitor maintenance. p2 Progenitors in the ventricular zone detected by irx3a (A) or nkx6.1 (B) were reduced in notch1a/notch1b/notch3 knockdown embryos (90%, irx3a, n=10; 80%, nkx6.1, n=10). Other single or double knockdown embryos did not show a striking reduction in irx3a (A) or nkx6.1 (B) expression compared with the notch1a/notch1b/notch3 knockdown embryos. All panels: side views of embryos at 24 hpf with anterior to the left, dorsal up. Dorsal and ventral borders of the neural tubes are shown by the dotted lines. Bar scale: 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
irx3a WT control Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO2-notch3 + MO3-notch1a + MO3-notch3 + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO2-notch3 + MO3-notch3 standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO3-notch1a + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch3 + MO3-notch1a + MO3-notch3 + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch3 + MO3-notch3 standard conditions Prim-5 spinal cord interneuron ISH
WT + MO3-notch1a + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
nkx6.1 WT control Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO2-notch3 + MO3-notch1a + MO3-notch3 + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO2-notch3 + MO3-notch3 standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch1b + MO3-notch1a + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch3 + MO3-notch1a + MO3-notch3 + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-notch3 + MO3-notch3 standard conditions Prim-5 spinal cord interneuron ISH
WT + MO3-notch1a + MO4-notch1a standard conditions Prim-5 spinal cord interneuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 391(2), Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M., Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination, 196-206, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.