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Okigawa et al., 2014 - Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination. Developmental Biology   391(2):196-206 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3 Mib, DeltaA, DeltaC, and DeltaD, but not Delta4 play important roles in V2 development. (A–E) V2a and V2b cells were identified as vsx1:GFP/Vsx2 double positive and vsx1:GFP/Scl double positive cells, respectively. Side views of embryos (control MO, deltaA/deltaC double MOs, deltaA/deltaD double MOs, deltaA/deltaC/deltaD triple MOs, or mib MO) at 24 hpf with anterior to the left, dorsal up. Bar scale: 50 µm. (F) V2a and V2b cell numbers per section, (G) total V2 cell number (V2a+V2b) per section, and (H) V2a-to-V2b ratio in mib and delta knockdown embryos. (F–H) Control MO, n=21; mib MO, n=4; deltaA MO, n=23; deltaC MO, n=4; deltaA MO, n=5; delta4/deltaA MOs, n=25; delta4/deltaC MOs, n=14; deltaA/deltaC MOs, n=27; deltaA/deltaD MOs, n=20; deltaA/deltaC/deltaD MOs, n=8. Asterisks indicate statistically significant differences relative to the control (p<0.01). Error bars, SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP nns5Tg control Prim-5 CiD IHC
Prim-5 VeLD IHC
nns5Tg + MO1-mib1 standard conditions Prim-5 CiD IHC
Prim-5 VeLD IHC
nns5Tg + MO2-dlc + MO2-dld + MO4-dla standard conditions Prim-5 CiD IHC
Prim-5 VeLD IHC
nns5Tg + MO2-dlc + MO4-dla standard conditions Prim-5 CiD IHC
Prim-5 VeLD IHC
nns5Tg + MO2-dld + MO4-dla standard conditions Prim-5 CiD IHC
Prim-5 VeLD IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nns5Tg + MO1-mib1 standard conditions Prim-5 CiD increased amount, abnormal
nns5Tg + MO2-dlc + MO2-dld + MO4-dla standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
nns5Tg + MO2-dlc + MO4-dla standard conditions Prim-5 CiD increased amount, abnormal
nns5Tg + MO2-dld + MO4-dla standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
nns5Tg + MO2-dll4 standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
nns5Tg + MO2-dll4 + MO4-dla standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
nns5Tg + MO4-dla standard conditions Prim-5 CiD increased amount, abnormal
Prim-5 VeLD increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 391(2), Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M., Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination, 196-206, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.