FIGURE

Fig. 2

ID

ZDB-FIG-140626-9

Publication

Okigawa et al., 2014 - Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination

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Fig. 2

DeltaA and DeltaD are redundant for p2 progenitor maintenance. (A, B) p2 progenitors were significantly reduced in deltaA/D double mutant embryos compared with any single or other double mutants. p2 Progenitors were detected by irx3a (A) or nkx6.1 (B). Sibling control (sib), deltaA mutant (dla^dmc72a), deltaD mutant (dld^tr33), deltaA/D double mutants (dla^dmc72a; dld^tr33), control morpholino injected embryo (con MO), deltaC morpholino injected embryo (dlc MO), deltaA/deltaC double morpholino injected embryo (dla; dlc MO), or deltaC/deltaD double morpholino injected embryo (dlc; dld MO). Left four panels in each row: side views of embryos at 24 hpf with anterior to the left, dorsal up. Dorsal and ventral borders of the neural tubes are shown by the dotted lines. Bar scale: 100 µm. Right two panels: transverse sections through the trunk region in embryos at 24 hpf. p2 Progenitor domains within the neural tubes (circled by white dotted lines) are indicated by red dashed outlines. Bar scale: 20 µm.

Expression Data

Genes:	irx3a nkx6.1
Fish:	WT dld^tr233/tr233 WT + MO2-dlc dla^{nkhspGFFDMC72AEt}; nkuasgfp1aTg WT + MO2-dlc + MO2-dld WT + MO2-dlc + MO4-dla dld^tr233/tr233; dla^{nkhspGFFDMC72AEt}
Knockdown Reagents:	MO2-dlc MO2-dld MO4-dla
Anatomical Term:	spinal cord interneuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 391(2), Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M., Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination, 196-206, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.