ZFIN ID: ZDB-FIG-140626-9
Okigawa et al., 2014 - Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination. Developmental Biology   391(2):196-206 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. 2 DeltaA and DeltaD are redundant for p2 progenitor maintenance. (A, B) p2 progenitors were significantly reduced in deltaA/D double mutant embryos compared with any single or other double mutants. p2 Progenitors were detected by irx3a (A) or nkx6.1 (B). Sibling control (sib), deltaA mutant (dladmc72a), deltaD mutant (dldtr33), deltaA/D double mutants (dladmc72a; dldtr33), control morpholino injected embryo (con MO), deltaC morpholino injected embryo (dlc MO), deltaA/deltaC double morpholino injected embryo (dla; dlc MO), or deltaC/deltaD double morpholino injected embryo (dlc; dld MO). Left four panels in each row: side views of embryos at 24 hpf with anterior to the left, dorsal up. Dorsal and ventral borders of the neural tubes are shown by the dotted lines. Bar scale: 100 µm. Right two panels: transverse sections through the trunk region in embryos at 24 hpf. p2 Progenitor domains within the neural tubes (circled by white dotted lines) are indicated by red dashed outlines. Bar scale: 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
irx3a WT control Prim-5 spinal cord interneuron ISH
WT standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc + MO2-dld standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc + MO4-dla standard conditions Prim-5 spinal cord interneuron ISH
dlankhspGFFDMC72AEt; nkuasgfp1aTg standard conditions Prim-5 spinal cord interneuron ISH
dldtr233/tr233 standard conditions Prim-5 spinal cord interneuron ISH
dldtr233/tr233; dlankhspGFFDMC72AEt standard conditions Prim-5 spinal cord interneuron ISH
nkx6.1 WT control Prim-5 spinal cord interneuron ISH
WT standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc + MO2-dld standard conditions Prim-5 spinal cord interneuron ISH
WT + MO2-dlc + MO4-dla standard conditions Prim-5 spinal cord interneuron ISH
dlankhspGFFDMC72AEt; nkuasgfp1aTg standard conditions Prim-5 spinal cord interneuron ISH
dldtr233/tr233 standard conditions Prim-5 spinal cord interneuron ISH
dldtr233/tr233; dlankhspGFFDMC72AEt standard conditions Prim-5 spinal cord interneuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 391(2), Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M., Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination, 196-206, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.